Protocol for using TRIBE to study RNA-protein interactions and nuclear organization in mammalian cells

Protocol for using TRIBE to study RNA-protein interactions and nuclear organization in mammalian cells

Summary: Targets of RNA-binding proteins discovered by editing (TRIBE) determines RNA-proteins interactions and nuclear organization with minimal false positives. We detail necessary steps for performing mammalian cell RBP-TRIBE to determine the targets of RNA-binding proteins and MS2-TRIBE to deter...

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Bibliographic Details
Main Authors: Jeetayu Biswas, Michael Rosbash, Robert H. Singer, Reazur Rahman
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Bioinformatics
Flow Cytometry/Mass Cytometry
Gene Expression
Genomics
Molecular Biology
RNAseq
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721003415

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http://www.sciencedirect.com/science/article/pii/S2666166721003415

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