Benchmark of 16S rRNA gene amplicon sequencing using Japanese gut microbiome data from the V1–V2 and V3–V4 primer sets

Benchmark of 16S rRNA gene amplicon sequencing using Japanese gut microbiome data from the V1–V2 and V3–V4 primer sets

Abstract Background 16S rRNA gene amplicon sequencing (16S analysis) is widely used to analyze microbiota with next-generation sequencing technologies. Here, we compared fecal 16S analysis data from 192 Japanese volunteers using the modified V1–V2 (V12) and the standard V3–V4 primer (V34) sets to op...

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Bibliographic Details
Main Authors: Shoichiro Kameoka, Daisuke Motooka, Satoshi Watanabe, Ryuichi Kubo, Nicolas Jung, Yuki Midorikawa, Natsuko O. Shinozaki, Yu Sawai, Aya K. Takeda, Shota Nakamura
Format: Article
Language:English
Published: BMC 2021-07-01
Series:BMC Genomics
Subjects:
Microbiota
16S rRNA
Next-generation sequencing
Online Access:https://doi.org/10.1186/s12864-021-07746-4

Internet

https://doi.org/10.1186/s12864-021-07746-4

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