Development of the Method to Produce Functionally Active Recombinant Streptavidin in Escherichia coli Cells

Streptavidin is a homotetrameric protein produced by Streptomyces avidinii, each subunit of which binds biotin (vitamin H), forming a stable complex (Kd = 10-15 М). Streptavidin-biotin coreaction is widely used in analytical systems, for targeted delivery of compounds, for affinity purification, etc...

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Bibliographic Details
Main Authors: Eugenia E. Bashmakova, Alexander N. Kudryavtsev, Ludmila A. Frank
Format: Article
Language:English
Published: Siberian Federal University 2020-06-01
Series:Журнал Сибирского федерального университета: Серия Биология
Subjects:
Online Access:http://elib.sfu-kras.ru/bitstream/2311/135353/1/08_Bashmakova.pdf