NK cell isolation from liver biopsies: phenotypic and functional analysis of low cell numbers by flow cytometry

NK cell isolation from liver biopsies: phenotypic and functional analysis of low cell numbers by flow cytometry

NK cells are considered to play a critical role in liver disease. However, the available numbers of intrahepatic lymphocytes (IHL) derived from liver biopsies (LB) for ex vivo analysis of intrahepatic NK cells is very limited; and the isolation method may hamper not only yields and viability, but al...

Full description

Bibliographic Details
Main Authors: Ning eLi, Gisella L. Puga Yung, Amandine ePradier, Christian eToso, Emiliano eGiostra, Isabelle eMorard, Laurent eSpahr, Jörg D. Seebach
Format: Article
Language:English
Published: Frontiers Media S.A. 2013-03-01
Series:Frontiers in Immunology
Subjects:
Flow Cytometry
human NK cells
liver biopsy
intrahepatic lymphocytes
collagenase
IFN- γ
Online Access:http://journal.frontiersin.org/Journal/10.3389/fimmu.2013.00061/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fimmu.2013.00061/full

Similar Items