Up-regulated miR-145 Expression Inhibits Porcine Preadipocytes Differentiation by Targeting IRS1

• Main Author: Yunxue Guo, Yaosheng Chen, Yun Zhang, Yue Zhang, Luxi Chen, Delin Mo
• Format: Article
• Language: English
• Published: Ivyspring International Publisher 2012-01-01
• Series: International Journal of Biological Sciences
• Online Access: http://www.biolsci.org/v08p1408.htm

<p>Generally, most miRNAs that were up-regulated during differentiation promoted adipogenesis, but our research indicated that up-regulation of miR-145 in porcine preadipocytes did not promote but inhibit adipogenesis. In this study, miR-145 was significantly up-regulated during porcine dedifferentiated fat (DFAT) cells differentiation. In miR-145 overexpressed DFAT cells, adipogenesis was inhibited and triglycerides accumulation was decreased after hormone stimulation (<i>P</i>&#60;0.05). Furthermore, up-regulation of miR-145 expression repressed induction of mRNA levels of adipogenic markers, such as <i>CCAAT/enhancer-binding protein &#945; </i>(<i>C/EBP&#945;</i>), and <i>peroxisome proliferator-activated receptor &#947;2 </i>(<i>PPAR&#947;2</i>). These effects caused by miR-145 overexpression were mediated by <i>Insulin receptor substrate 1</i> (<i>IRS1</i>) as a mechanism. These data suggested that induced miR-145 expression during differentiation could inhibit adipogenesis by targeting IRS1, and miR-145 may be novel agent for adipose tissue engineering.</p>