A practical guide to build de-novo assemblies for single tissues of non-model organisms: the example of a Neotropical frog

Whole genome sequencing (WGS) is a very valuable resource to understand the evolutionary history of poorly known species. However, in organisms with large genomes, as most amphibians, WGS is still excessively challenging and transcriptome sequencing (RNA-seq) represents a cost-effective tool to expl...

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Bibliographic Details
Main Authors: Santiago Montero-Mendieta, Manfred Grabherr, Henrik Lantz, Ignacio De la Riva, Jennifer A. Leonard, Matthew T. Webster, Carles Vilà
Format: Article
Language:English
Published: PeerJ Inc. 2017-09-01
Series:PeerJ
Subjects:
Online Access:https://peerj.com/articles/3702.pdf