Genome Engineering in Plant Using an Efficient CRISPR-xCas9 Toolset With an Expanded PAM Compatibility

Genome Engineering in Plant Using an Efficient CRISPR-xCas9 Toolset With an Expanded PAM Compatibility

The CRISPR-Cas9 system enables simple, rapid, and effective genome editing in many species. Nevertheless, the requirement of an NGG protospacer adjacent motif (PAM) for the widely used canonical Streptococcus pyogenes Cas9 (SpCas9) limits the potential target sites. The xCas9, an engineered SpCas9 v...

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Bibliographic Details
Main Authors: Chengwei Zhang, Guiting Kang, Xinxiang Liu, Si Zhao, Shuang Yuan, Lu Li, Yongxing Yang, Feipeng Wang, Xiang Zhang, Jinxiao Yang
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-12-01
Series:Frontiers in Genome Editing
Subjects:
genome editing
xCas9
cytosine base editor
tRNA-sgRNA
rice
Online Access:https://www.frontiersin.org/articles/10.3389/fgeed.2020.618385/full

Internet

https://www.frontiersin.org/articles/10.3389/fgeed.2020.618385/full

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