Silencing of PSMC2 inhibits development and metastasis of prostate cancer through regulating proliferation, apoptosis and migration
Abstract Background Prostate cancer is the most common malignant tumor of male genitourinary system, molecular mechanism of which is still not clear. PSMC2 (proteasome 26S subunit ATPase 2) is a key member of the 19S regulatory subunit of 26S proteasome, whose relationship with prostate cancer is ra...
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doaj-6a1b24aac3cc4ea89a4201aa3bed4e272021-05-02T11:21:28ZengBMCCancer Cell International1475-28672021-04-0121111210.1186/s12935-021-01934-8Silencing of PSMC2 inhibits development and metastasis of prostate cancer through regulating proliferation, apoptosis and migrationQingke Chen0Lingmin Fu1Jieping Hu2Guanghua Guo3An Xie4Department of Urology, First Affiliated Hospital of Nanchang UniversityJiangxi Health Vocational CollegeDepartment of Urology, First Affiliated Hospital of Nanchang UniversityDepartment of Burns, First Affiliated Hospital of Nanchang UniversityInstitute of Urology, First Affiliated Hospital of Nanchang UniversityAbstract Background Prostate cancer is the most common malignant tumor of male genitourinary system, molecular mechanism of which is still not clear. PSMC2 (proteasome 26S subunit ATPase 2) is a key member of the 19S regulatory subunit of 26S proteasome, whose relationship with prostate cancer is rarely studied. Methods Here, expression of PSMC2 in tumor tissues or cells of prostate cancer was detected by qPCR, western blotting and immunohistochemical analysis. The effects of PSMC2 knockdown on cell proliferation, colony formation, cell migration, cell cycle and apoptosis were assessed by Celigo cell counting assay, colony formation assay, wound-healing assay, Transwell assay and flow cytometry, respectively. The influence of PSMC2 knockdown on tumor growth in vivo was evaluated by mice xenograft models. Results The results demonstrated that PSMC2 was upregulated in tumor tissues of prostate cancer and its high expression was significantly associated with advanced Gleason grade and higher Gleason score. Knockdown of PSMC2 could inhibited cell proliferation, colony formation and cell migration of prostate cancer cells, while promoting cell apoptosis and cell cycle arrest. The suppression of tumor growth in vivo by PSMC2 knockdown was also showed by using mice xenograft models. Moreover, the regulation of prostate cancer by PSMC2 may be mediated by Akt/Cyclin D1/CDK6 signaling pathway. Conclusions Therefore, our studies suggested that PSMC2 may act as a tumor promotor in the development and progression of prostate cancer, and could be considered as a novel therapeutic target for prostate cancer treatment.https://doi.org/10.1186/s12935-021-01934-8Prostate cancerPSMC2Cell proliferationCell apoptosisAkt pathway |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qingke Chen Lingmin Fu Jieping Hu Guanghua Guo An Xie |
spellingShingle |
Qingke Chen Lingmin Fu Jieping Hu Guanghua Guo An Xie Silencing of PSMC2 inhibits development and metastasis of prostate cancer through regulating proliferation, apoptosis and migration Cancer Cell International Prostate cancer PSMC2 Cell proliferation Cell apoptosis Akt pathway |
author_facet |
Qingke Chen Lingmin Fu Jieping Hu Guanghua Guo An Xie |
author_sort |
Qingke Chen |
title |
Silencing of PSMC2 inhibits development and metastasis of prostate cancer through regulating proliferation, apoptosis and migration |
title_short |
Silencing of PSMC2 inhibits development and metastasis of prostate cancer through regulating proliferation, apoptosis and migration |
title_full |
Silencing of PSMC2 inhibits development and metastasis of prostate cancer through regulating proliferation, apoptosis and migration |
title_fullStr |
Silencing of PSMC2 inhibits development and metastasis of prostate cancer through regulating proliferation, apoptosis and migration |
title_full_unstemmed |
Silencing of PSMC2 inhibits development and metastasis of prostate cancer through regulating proliferation, apoptosis and migration |
title_sort |
silencing of psmc2 inhibits development and metastasis of prostate cancer through regulating proliferation, apoptosis and migration |
publisher |
BMC |
series |
Cancer Cell International |
issn |
1475-2867 |
publishDate |
2021-04-01 |
description |
Abstract Background Prostate cancer is the most common malignant tumor of male genitourinary system, molecular mechanism of which is still not clear. PSMC2 (proteasome 26S subunit ATPase 2) is a key member of the 19S regulatory subunit of 26S proteasome, whose relationship with prostate cancer is rarely studied. Methods Here, expression of PSMC2 in tumor tissues or cells of prostate cancer was detected by qPCR, western blotting and immunohistochemical analysis. The effects of PSMC2 knockdown on cell proliferation, colony formation, cell migration, cell cycle and apoptosis were assessed by Celigo cell counting assay, colony formation assay, wound-healing assay, Transwell assay and flow cytometry, respectively. The influence of PSMC2 knockdown on tumor growth in vivo was evaluated by mice xenograft models. Results The results demonstrated that PSMC2 was upregulated in tumor tissues of prostate cancer and its high expression was significantly associated with advanced Gleason grade and higher Gleason score. Knockdown of PSMC2 could inhibited cell proliferation, colony formation and cell migration of prostate cancer cells, while promoting cell apoptosis and cell cycle arrest. The suppression of tumor growth in vivo by PSMC2 knockdown was also showed by using mice xenograft models. Moreover, the regulation of prostate cancer by PSMC2 may be mediated by Akt/Cyclin D1/CDK6 signaling pathway. Conclusions Therefore, our studies suggested that PSMC2 may act as a tumor promotor in the development and progression of prostate cancer, and could be considered as a novel therapeutic target for prostate cancer treatment. |
topic |
Prostate cancer PSMC2 Cell proliferation Cell apoptosis Akt pathway |
url |
https://doi.org/10.1186/s12935-021-01934-8 |
work_keys_str_mv |
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1721492294540984320 |