Similarities and differences in the biochemical and enzymological properties of the four isomaltases from Saccharomyces cerevisiae

Similarities and differences in the biochemical and enzymological properties of the four isomaltases from Saccharomyces cerevisiae

The yeast Saccharomyces cerevisiae IMA multigene family encodes four isomaltases sharing high sequence identity from 65% to 99%. Here, we explore their functional diversity, with exhaustive in-vitro characterization of their enzymological and biochemical properties. The four isoenzymes exhibited a p...

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Bibliographic Details
Main Authors: Xu Deng, Marjorie Petitjean, Marie-Ange Teste, Wafa Kooli, Samuel Tranier, Jean Marie François, Jean-Luc Parrou
Format: Article
Language:English
Published: Wiley 2014-01-01
Series:FEBS Open Bio
Subjects:
Yeast
α-Glucosidase
Isomaltase
Isomaltose
Maltose
Substrate ambiguity
Substrate inhibition
Thermostability
Proline substitution
Online Access:http://www.sciencedirect.com/science/article/pii/S2211546314000151

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http://www.sciencedirect.com/science/article/pii/S2211546314000151

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