Toward a cell-free hydantoinase process: screening for expression optimization and one-step purification as well as immobilization of hydantoinase and carbamoylase

Toward a cell-free hydantoinase process: screening for expression optimization and one-step purification as well as immobilization of hydantoinase and carbamoylase

Abstract The hydantoinase process is applied for the industrial synthesis of optically pure amino acids via whole cell biocatalysis, providing a simple and well-established method to obtain the catalyst. Nevertheless, whole cell approaches also bear disadvantages like intracellular degradation react...

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Bibliographic Details
Main Authors: Christin Slomka, Georg Paris Späth, Phillip Lemke, Marc Skoupi, Christof M. Niemeyer, Christoph Syldatk, Jens Rudat
Format: Article
Language:English
Published: SpringerOpen 2017-06-01
Series:AMB Express
Subjects:
Amino acids
Enzyme catalysis
Hydantoinase process
Protein purification
Enzyme immobilization
Magnetic beads
Online Access:http://link.springer.com/article/10.1186/s13568-017-0420-3

Internet

http://link.springer.com/article/10.1186/s13568-017-0420-3

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