New Peptide-Conjugated Chlorin-Type Photosensitizer Targeting Neuropilin-1 for Anti-Vascular Targeted Photodynamic Therapy
Photodynamic therapy (PDT) is a cancer treatment modality that requires three components, namely light, dioxygen and a photosensitizing agent. After light excitation, the photosensitizer (PS) in its excited state transfers its energy to oxygen, which leads to photooxidation reactions. In order to im...
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doaj-6a5a90d93bb04ab4ba926db04054b4232020-11-25T01:08:00ZengMDPI AGInternational Journal of Molecular Sciences1422-00672015-10-011610240592408010.3390/ijms161024059ijms161024059New Peptide-Conjugated Chlorin-Type Photosensitizer Targeting Neuropilin-1 for Anti-Vascular Targeted Photodynamic TherapyEzatul Ezleen Kamarulzaman0Amirah Mohd Gazzali1Samir Acherar2Céline Frochot3Muriel Barberi-Heyob4Cédric Boura5Patrick Chaimbault6Estelle Sibille7Habibah A. Wahab8Régis Vanderesse9LCPM UMR 7375, CNRS, ENSIC, 1 rue Grandville, BP 20451-54001 Nancy Cedex, FranceLCPM UMR 7375, CNRS, ENSIC, 1 rue Grandville, BP 20451-54001 Nancy Cedex, FranceLCPM UMR 7375, CNRS, ENSIC, 1 rue Grandville, BP 20451-54001 Nancy Cedex, FranceLRGP, UMR 7274, CNRS, ENSIC, 1 rue Grandville, BP 20451-54001 Nancy Cedex, FranceCRAN, UMR 7039, Université de Lorraine, Campus Sciences, BP 70239-54506 Vandoeuvre Cedex, FranceCRAN, UMR 7039, Université de Lorraine, Campus Sciences, BP 70239-54506 Vandoeuvre Cedex, FranceSRSMC, UMR 7565 ICPM, Université de Lorraine, 1 boulevard Arago, 57078 Metz Cedex 3, FranceLCP-A2MC, EA 4632, ICPM, 1 boulevard Arago, 57078 Metz Cedex 3, FranceSchool of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Penang, MalaysiaLCPM UMR 7375, CNRS, ENSIC, 1 rue Grandville, BP 20451-54001 Nancy Cedex, FrancePhotodynamic therapy (PDT) is a cancer treatment modality that requires three components, namely light, dioxygen and a photosensitizing agent. After light excitation, the photosensitizer (PS) in its excited state transfers its energy to oxygen, which leads to photooxidation reactions. In order to improve the selectivity of the treatment, research has focused on the design of PS covalently attached to a tumor-targeting moiety. In this paper, we describe the synthesis and the physico-chemical and photophysical properties of six new peptide-conjugated photosensitizers designed for targeting the neuropilin-1 (NRP-1) receptor. We chose a TPC (5-(4-carboxyphenyl)-10,15, 20-triphenyl chlorine as photosensitizer, coupled via three different spacers (aminohexanoic acid, 1-amino-3,6-dioxaoctanoic acid, and 1-amino-9-aza-3,6,12,15-tetraoxa-10-on-heptadecanoic acid) to two different peptides (DKPPR and TKPRR). The affinity towards the NRP-1 receptor of the conjugated chlorins was evaluated along with in vitro and in vivo stability levels. The tissue concentration of the TPC-conjugates in animal model shows good distribution, especially for the DKPPR conjugates. The novel peptide–PS conjugates proposed in this study were proven to have potential to be further developed as future NRP-1 targeting photodynamic therapy agent.http://www.mdpi.com/1422-0067/16/10/24059photodynamic therapypeptide targeted photosensitizerneuropilin-1in vivo stability |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ezatul Ezleen Kamarulzaman Amirah Mohd Gazzali Samir Acherar Céline Frochot Muriel Barberi-Heyob Cédric Boura Patrick Chaimbault Estelle Sibille Habibah A. Wahab Régis Vanderesse |
spellingShingle |
Ezatul Ezleen Kamarulzaman Amirah Mohd Gazzali Samir Acherar Céline Frochot Muriel Barberi-Heyob Cédric Boura Patrick Chaimbault Estelle Sibille Habibah A. Wahab Régis Vanderesse New Peptide-Conjugated Chlorin-Type Photosensitizer Targeting Neuropilin-1 for Anti-Vascular Targeted Photodynamic Therapy International Journal of Molecular Sciences photodynamic therapy peptide targeted photosensitizer neuropilin-1 in vivo stability |
author_facet |
Ezatul Ezleen Kamarulzaman Amirah Mohd Gazzali Samir Acherar Céline Frochot Muriel Barberi-Heyob Cédric Boura Patrick Chaimbault Estelle Sibille Habibah A. Wahab Régis Vanderesse |
author_sort |
Ezatul Ezleen Kamarulzaman |
title |
New Peptide-Conjugated Chlorin-Type Photosensitizer Targeting Neuropilin-1 for Anti-Vascular Targeted Photodynamic Therapy |
title_short |
New Peptide-Conjugated Chlorin-Type Photosensitizer Targeting Neuropilin-1 for Anti-Vascular Targeted Photodynamic Therapy |
title_full |
New Peptide-Conjugated Chlorin-Type Photosensitizer Targeting Neuropilin-1 for Anti-Vascular Targeted Photodynamic Therapy |
title_fullStr |
New Peptide-Conjugated Chlorin-Type Photosensitizer Targeting Neuropilin-1 for Anti-Vascular Targeted Photodynamic Therapy |
title_full_unstemmed |
New Peptide-Conjugated Chlorin-Type Photosensitizer Targeting Neuropilin-1 for Anti-Vascular Targeted Photodynamic Therapy |
title_sort |
new peptide-conjugated chlorin-type photosensitizer targeting neuropilin-1 for anti-vascular targeted photodynamic therapy |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2015-10-01 |
description |
Photodynamic therapy (PDT) is a cancer treatment modality that requires three components, namely light, dioxygen and a photosensitizing agent. After light excitation, the photosensitizer (PS) in its excited state transfers its energy to oxygen, which leads to photooxidation reactions. In order to improve the selectivity of the treatment, research has focused on the design of PS covalently attached to a tumor-targeting moiety. In this paper, we describe the synthesis and the physico-chemical and photophysical properties of six new peptide-conjugated photosensitizers designed for targeting the neuropilin-1 (NRP-1) receptor. We chose a TPC (5-(4-carboxyphenyl)-10,15, 20-triphenyl chlorine as photosensitizer, coupled via three different spacers (aminohexanoic acid, 1-amino-3,6-dioxaoctanoic acid, and 1-amino-9-aza-3,6,12,15-tetraoxa-10-on-heptadecanoic acid) to two different peptides (DKPPR and TKPRR). The affinity towards the NRP-1 receptor of the conjugated chlorins was evaluated along with in vitro and in vivo stability levels. The tissue concentration of the TPC-conjugates in animal model shows good distribution, especially for the DKPPR conjugates. The novel peptide–PS conjugates proposed in this study were proven to have potential to be further developed as future NRP-1 targeting photodynamic therapy agent. |
topic |
photodynamic therapy peptide targeted photosensitizer neuropilin-1 in vivo stability |
url |
http://www.mdpi.com/1422-0067/16/10/24059 |
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