Attenuation of Virulent Porcine Reproductive and Respiratory Syndrome Virus Strain CH-1a and Genetic Variation of ORF5 Gene
To develop a modified live vaccine (MLV) against porcine reproductive and respiratory syndrome virus (PRRSV), virulent CH-1a strain was attenuated by serial passages up to 130 passage (P130) in Marc-145 cells. The virulence and immune efficacy of the attenuated CH-1a were evaluated in pigs. The resu...
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doaj-6a6a03a953904696b5b560499043b2fd2021-06-07T06:48:10ZengElsevierJournal of Integrative Agriculture2095-31192012-12-01111220352042Attenuation of Virulent Porcine Reproductive and Respiratory Syndrome Virus Strain CH-1a and Genetic Variation of ORF5 GeneXue-hui CAI0Guo-jun WU1Yong-gang LIU2Guang-qing LIU3Wen-da SHI4Shu-jie WANG5Ping MA6Cheng-jun LI7Wen-yu HAN8College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, P.R. China; State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, P.R. China; CAI Xue-hui, Tel: +86-451-51997183State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, P.R. ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, P.R. ChinaDivision of Avian Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, P.R. ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, P.R. ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, P.R. ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, P.R. ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, P.R. ChinaCollege of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, P.R. China; Correspondence HAN Wen-yu, Tel: +86-431-87836001, Fax: +86-431-87836029To develop a modified live vaccine (MLV) against porcine reproductive and respiratory syndrome virus (PRRSV), virulent CH-1a strain was attenuated by serial passages up to 130 passage (P130) in Marc-145 cells. The virulence and immune efficacy of the attenuated CH-1a were evaluated in pigs. The results showed that animals inoculated with P130 did not develop any clinical sign of the disease, but produced rapid and effective humoral immune responses against PRRSV challenge, indicating that attenuated CH-1a P130 is the candidate as the effective vaccine against PRRSV. To define the potential mutations in the attenuated CH-1a genome, we sequenced and analyzed the ORF5 gene of CH-1a strain of different passages (P39, P55, P65, P70, P85, P100, P115, P120, P125, and P130) and found that three mutations (C5Y, H38Q and L146Q) which may be related with the attenuation of CH-1a. In addition, we also found a unique restriction enzyme site (TspEI) in the ORF5 gene of attenuated CH-1a, which can be used as a genetic marker to distinguish original and attenuated CH-1a.http://www.sciencedirect.com/science/article/pii/S2095311912604614attenuationporcine reproductive and respiratory syndrome virusgenetic variationORF5 gene |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xue-hui CAI Guo-jun WU Yong-gang LIU Guang-qing LIU Wen-da SHI Shu-jie WANG Ping MA Cheng-jun LI Wen-yu HAN |
spellingShingle |
Xue-hui CAI Guo-jun WU Yong-gang LIU Guang-qing LIU Wen-da SHI Shu-jie WANG Ping MA Cheng-jun LI Wen-yu HAN Attenuation of Virulent Porcine Reproductive and Respiratory Syndrome Virus Strain CH-1a and Genetic Variation of ORF5 Gene Journal of Integrative Agriculture attenuation porcine reproductive and respiratory syndrome virus genetic variation ORF5 gene |
author_facet |
Xue-hui CAI Guo-jun WU Yong-gang LIU Guang-qing LIU Wen-da SHI Shu-jie WANG Ping MA Cheng-jun LI Wen-yu HAN |
author_sort |
Xue-hui CAI |
title |
Attenuation of Virulent Porcine Reproductive and Respiratory Syndrome Virus Strain CH-1a and Genetic Variation of ORF5 Gene |
title_short |
Attenuation of Virulent Porcine Reproductive and Respiratory Syndrome Virus Strain CH-1a and Genetic Variation of ORF5 Gene |
title_full |
Attenuation of Virulent Porcine Reproductive and Respiratory Syndrome Virus Strain CH-1a and Genetic Variation of ORF5 Gene |
title_fullStr |
Attenuation of Virulent Porcine Reproductive and Respiratory Syndrome Virus Strain CH-1a and Genetic Variation of ORF5 Gene |
title_full_unstemmed |
Attenuation of Virulent Porcine Reproductive and Respiratory Syndrome Virus Strain CH-1a and Genetic Variation of ORF5 Gene |
title_sort |
attenuation of virulent porcine reproductive and respiratory syndrome virus strain ch-1a and genetic variation of orf5 gene |
publisher |
Elsevier |
series |
Journal of Integrative Agriculture |
issn |
2095-3119 |
publishDate |
2012-12-01 |
description |
To develop a modified live vaccine (MLV) against porcine reproductive and respiratory syndrome virus (PRRSV), virulent CH-1a strain was attenuated by serial passages up to 130 passage (P130) in Marc-145 cells. The virulence and immune efficacy of the attenuated CH-1a were evaluated in pigs. The results showed that animals inoculated with P130 did not develop any clinical sign of the disease, but produced rapid and effective humoral immune responses against PRRSV challenge, indicating that attenuated CH-1a P130 is the candidate as the effective vaccine against PRRSV. To define the potential mutations in the attenuated CH-1a genome, we sequenced and analyzed the ORF5 gene of CH-1a strain of different passages (P39, P55, P65, P70, P85, P100, P115, P120, P125, and P130) and found that three mutations (C5Y, H38Q and L146Q) which may be related with the attenuation of CH-1a. In addition, we also found a unique restriction enzyme site (TspEI) in the ORF5 gene of attenuated CH-1a, which can be used as a genetic marker to distinguish original and attenuated CH-1a. |
topic |
attenuation porcine reproductive and respiratory syndrome virus genetic variation ORF5 gene |
url |
http://www.sciencedirect.com/science/article/pii/S2095311912604614 |
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