Neutrophils Do Not Express IL-17A in the Context of Acute Oropharyngeal Candidiasis
IL-17 protects against pathogens by acting on nonhematopoietic cells to induce neutrophil recruitment through upregulation of chemokines and G-CSF. IL-17- and Th17-deficient humans and mice are susceptible to mucosal Candida albicans infections, linked to impaired neutrophil responses. IL-17 produc...
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doaj-6b0f0747f6b241838b60f735fd674fae2020-11-25T00:01:43ZengMDPI AGPathogens2076-08172015-07-014355957210.3390/pathogens4030559pathogens4030559Neutrophils Do Not Express IL-17A in the Context of Acute Oropharyngeal CandidiasisAnna R. Huppler0Akash H. Verma1Heather R. Conti2Sarah L. Gaffen3Children's Hospital of Pittsburgh of UPMC, Department of Pediatrics, Pittsburgh, PA 15224, USADepartment of Medicine, Division of Rheumatology & Clinical Immunology, University of Pittsburgh, BST S702, 200 Lothrop Street, Pittsburgh, PA 15261, USADepartment of Medicine, Division of Rheumatology & Clinical Immunology, University of Pittsburgh, BST S702, 200 Lothrop Street, Pittsburgh, PA 15261, USADepartment of Medicine, Division of Rheumatology & Clinical Immunology, University of Pittsburgh, BST S702, 200 Lothrop Street, Pittsburgh, PA 15261, USAIL-17 protects against pathogens by acting on nonhematopoietic cells to induce neutrophil recruitment through upregulation of chemokines and G-CSF. IL-17- and Th17-deficient humans and mice are susceptible to mucosal Candida albicans infections, linked to impaired neutrophil responses. IL-17 production is traditionally associated with CD4+ Th17 cells. However, IL-17 is also expressed during innate responses to facilitate rapid pathogen clearance. Innate IL-17-expressing cells include various lymphocyte-type subsets, including ILC3, NKT, γδ-T and “natural” Th17 (nTh17) cells. Some reports suggest that neutrophils can express IL-17 during fungal infections. Here, we asked whether neutrophils serve as a source of IL-17 during acute oropharyngeal candidiasis (OPC) using an IL-17A fate-tracking reporter mouse. Mice were subjected to OPC for two days, and oral tissue was analyzed by flow cytometry. IL-17A was expressed by γδ-T cells and TCRβ+ natural Th17 (nTh17) cells, as recently reported. Although infiltrating neutrophils were recruited to the tongue following infection, they did not express the IL-17A reporter. Moreover, neutrophil-depleted mice exhibited normal transcription of both Il17a and downstream IL-17-dependent gene targets after Candida challenge. Thus, in acute OPC, neutrophils are not a measurable source of IL-17 production, nor are they necessary to trigger IL-17-dependent gene expression, although they are essential for ultimate pathogen control.http://www.mdpi.com/2076-0817/4/3/559IL-17Th17Candida albicansneutrophilsfungal infectionoral candidiasisinnate lymphocyteTCRγδ-T cellmice |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Anna R. Huppler Akash H. Verma Heather R. Conti Sarah L. Gaffen |
spellingShingle |
Anna R. Huppler Akash H. Verma Heather R. Conti Sarah L. Gaffen Neutrophils Do Not Express IL-17A in the Context of Acute Oropharyngeal Candidiasis Pathogens IL-17 Th17 Candida albicans neutrophils fungal infection oral candidiasis innate lymphocyte TCR γδ-T cell mice |
author_facet |
Anna R. Huppler Akash H. Verma Heather R. Conti Sarah L. Gaffen |
author_sort |
Anna R. Huppler |
title |
Neutrophils Do Not Express IL-17A in the Context of Acute Oropharyngeal Candidiasis |
title_short |
Neutrophils Do Not Express IL-17A in the Context of Acute Oropharyngeal Candidiasis |
title_full |
Neutrophils Do Not Express IL-17A in the Context of Acute Oropharyngeal Candidiasis |
title_fullStr |
Neutrophils Do Not Express IL-17A in the Context of Acute Oropharyngeal Candidiasis |
title_full_unstemmed |
Neutrophils Do Not Express IL-17A in the Context of Acute Oropharyngeal Candidiasis |
title_sort |
neutrophils do not express il-17a in the context of acute oropharyngeal candidiasis |
publisher |
MDPI AG |
series |
Pathogens |
issn |
2076-0817 |
publishDate |
2015-07-01 |
description |
IL-17 protects against pathogens by acting on nonhematopoietic cells to induce neutrophil recruitment through upregulation of chemokines and G-CSF. IL-17- and Th17-deficient humans and mice are susceptible to mucosal Candida albicans infections, linked to impaired neutrophil responses. IL-17 production is traditionally associated with CD4+ Th17 cells. However, IL-17 is also expressed during innate responses to facilitate rapid pathogen clearance. Innate IL-17-expressing cells include various lymphocyte-type subsets, including ILC3, NKT, γδ-T and “natural” Th17 (nTh17) cells. Some reports suggest that neutrophils can express IL-17 during fungal infections. Here, we asked whether neutrophils serve as a source of IL-17 during acute oropharyngeal candidiasis (OPC) using an IL-17A fate-tracking reporter mouse. Mice were subjected to OPC for two days, and oral tissue was analyzed by flow cytometry. IL-17A was expressed by γδ-T cells and TCRβ+ natural Th17 (nTh17) cells, as recently reported. Although infiltrating neutrophils were recruited to the tongue following infection, they did not express the IL-17A reporter. Moreover, neutrophil-depleted mice exhibited normal transcription of both Il17a and downstream IL-17-dependent gene targets after Candida challenge. Thus, in acute OPC, neutrophils are not a measurable source of IL-17 production, nor are they necessary to trigger IL-17-dependent gene expression, although they are essential for ultimate pathogen control. |
topic |
IL-17 Th17 Candida albicans neutrophils fungal infection oral candidiasis innate lymphocyte TCR γδ-T cell mice |
url |
http://www.mdpi.com/2076-0817/4/3/559 |
work_keys_str_mv |
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