Two Methods for Increased Specificity and Sensitivity in Loop-Mediated Isothermal Amplification

The technique of loop-mediated isothermal amplification (LAMP) utilizes four (or six) primers targeting six (or eight) regions within a fairly small segment of a genome for amplification, with concentration higher than that used in traditional PCR methods. The high concentrations of primers used le...

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Bibliographic Details
Main Authors: De-Guo Wang, Jeffrey D. Brewster, Moushumi Paul, Peggy M. Tomasula
Format: Article
Language:English
Published: MDPI AG 2015-04-01
Series:Molecules
Subjects:
Online Access:http://www.mdpi.com/1420-3049/20/4/6048