Automated Cell-Free Multiprotein Synthesis Facilitates the Identification of a Secretory, Oligopeptide Elicitor-Like, Immunoreactive Protein of the Oomycete Pythium insidiosum

Automated Cell-Free Multiprotein Synthesis Facilitates the Identification of a Secretory, Oligopeptide Elicitor-Like, Immunoreactive Protein of the Oomycete Pythium insidiosum

Technical limitations of conventional biotechnological methods (i.e., genetic engineering and protein synthesis) prevent extensive functional studies of the massive amounts of genetic information available today. We employed a cell-free protein synthesis system to rapidly and simultaneously generate...

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Bibliographic Details
Main Authors: Pattarana Sae-Chew, Thidarat Rujirawat, Yothin Kumsang, Penpan Payattikul, Tassanee Lohnoo, Wanta Yingyong, Chalisa Jaturapaktrarak, Tiwa Rotchanapreeda, Onrapak Reamtong, Tanawut Srisuk, Weerayuth Kittichotirat, Theerapong Krajaejun
Format: Article
Language:English
Published: American Society for Microbiology 2020-05-01
Series:mSystems
Subjects:
pythium insidiosum
cell-free protein synthesis
evolution
immunoreactive protein
oomycete
pythiosis
oligopeptide elicitor
Online Access:https://doi.org/10.1128/mSystems.00196-20

Internet

https://doi.org/10.1128/mSystems.00196-20

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