Enhanced Heterologous Production of Glycosyltransferase UGT76G1 by Co-Expression of Endogenous <i>prpD</i> and <i>malK</i> in <i>Escherichia coli</i> and Its Transglycosylation Application in Production of Rebaudioside

Enhanced Heterologous Production of Glycosyltransferase UGT76G1 by Co-Expression of Endogenous <i>prpD</i> and <i>malK</i> in <i>Escherichia coli</i> and Its Transglycosylation Application in Production of Rebaudioside

Steviol glycosides (SGs) with zero calories and high-intensity sweetness are the best substitutes of sugar for the human diet. Uridine diphosphate dependent glycosyltransferase (UGT) UGT76G1, as a key enzyme for the biosynthesis of SGs with a low heterologous expression level, hinders its applicatio...

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Bibliographic Details
Main Authors: Wenju Shu, Hongchen Zheng, Xiaoping Fu, Jie Zhen, Ming Tan, Jianyong Xu, Xingya Zhao, Shibin Yang, Hui Song, Yanhe Ma
Format: Article
Language:English
Published: MDPI AG 2020-08-01
Series:International Journal of Molecular Sciences
Subjects:
steviol glycosides
fusion partner
efficient <i>E. coli</i> expression system
<i>prpD</i>
<i>malK</i>
co-expression
Online Access:https://www.mdpi.com/1422-0067/21/16/5752

Internet

https://www.mdpi.com/1422-0067/21/16/5752

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