A duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains

Abstract INTRODUCTION: This study aimed to develop a duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains. METHODS: Primers were designed to target the rrs (LG1/LG2) and ligB (LP1/LP2) genes to confirm the presence of the Leptospira genus and the pathogenic spe...

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Bibliographic Details
Main Authors: Douadi Benacer, Siti Nursheena Mohd Zain, John W. Lewis, Mohd Khairul Nizam Mohd Khalid, Kwai Lin Thong
Format: Article
Language:English
Published: Sociedade Brasileira de Medicina Tropical (SBMT)
Series:Revista da Sociedade Brasileira de Medicina Tropical
Subjects:
PCR
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822017000200239&lng=en&tlng=en