Nanopore long-read RNA-seq and absolute quantification delineate transcription dynamics in early embryo development of an insect pest

Abstract The olive fruit fly, Bactrocera oleae, is the most important pest for the olive fruit but lacks adequate transcriptomic characterization that could aid in molecular control approaches. We apply nanopore long-read RNA-seq with internal RNA standards allowing absolute transcript quantificatio...

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Bibliographic Details
Main Authors: Anthony Bayega, Spyros Oikonomopoulos, Maria-Eleni Gregoriou, Konstantina T. Tsoumani, Antonis Giakountis, Yu Chang Wang, Kostas D. Mathiopoulos, Jiannis Ragoussis
Format: Article
Language:English
Published: Nature Publishing Group 2021-04-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-86753-7