Cold Formalin Fixation Guarantees DNA Integrity in Formalin Fixed Paraffin Embedded Tissues: Premises for a Better Quality of Diagnostic and Experimental Pathology With a Specific Impact on Breast Cancer

Cold Formalin Fixation Guarantees DNA Integrity in Formalin Fixed Paraffin Embedded Tissues: Premises for a Better Quality of Diagnostic and Experimental Pathology With a Specific Impact on Breast Cancer

Formalin fixation and paraffin embedding (FFPE) represent the standard method to preserve tissue specimens for diagnostic pathology, however formalin fixation induces severe fragmentation of nucleic acids. We investigated whether formalin fixation at 4°C could preserve DNA integrity in FFPE specimen...

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Bibliographic Details
Main Authors: Enrico Berrino, Laura Annaratone, Umberto Miglio, Elena Maldi, Chiara Piccinelli, Erica Peano, Davide Balmativola, Paola Cassoni, Alberto Pisacane, Ivana Sarotto, Tiziana Venesio, Anna Sapino, Caterina Marchiò
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-02-01
Series:Frontiers in Oncology
Subjects:
fixation
cold formalin
DNA fragmentation
molecular diagnostics
breast cancer
diagnostic accuracy
Online Access:https://www.frontiersin.org/article/10.3389/fonc.2020.00173/full

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https://www.frontiersin.org/article/10.3389/fonc.2020.00173/full

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