Propidium monoazide integrated with qPCR enables the detection and enumeration of infectious enteric RNA and DNA viruses in clam and fermented sausages

Propidium monoazide integrated with qPCR enables the detection and enumeration of infectious enteric RNA and DNA viruses in clam and fermented sausages

The increase of foodborne viral outbreaks highlights the need for a rapid and sensitive method for the prediction of viral infectivity in food samples. This study assesses the use of propidium monoazide (PMA) coupled with real-time PCR methods (RT-qPCR or qPCR for RNA or DNA viruses, respectively) i...

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Bibliographic Details
Main Authors: Narciso M Quijada, Gislaine Fongaro, Célia Regina Monte Barardi, Marta Hernandez, David Rodriguez-Lazaro
Format: Article
Language:English
Published: Frontiers Media S.A. 2016-12-01
Series:Frontiers in Microbiology
Subjects:
enteric viruses
clam
Infectivity prediction
PMA-PCR
fermented sausage.
Online Access:http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.02008/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.02008/full

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