Transformation of coffee (Coffea Arabica L. cv. Catimor) with the cry1ac gene by biolistic, without the use of markers

The transformation of coffee plantlets with the cry1ac gene of Bacillus thuringiensis was achieved by biolistic using either the whole pUBC plasmid or only the ubi-cry1ac-nos genetic cassette. The cry1ac gene was inserted into coffee plants in order to confer resistance to the leaf miner Leucoptera...

Full description

Bibliographic Details
Main Authors: Z De Guglielmo-Cróquer, I. Altosaar, M. Zaidi, A. Menéndez-Yuffá
Format: Article
Language:English
Published: Instituto Internacional de Ecologia
Series:Brazilian Journal of Biology
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842010000200022&lng=en&tlng=en