MPRAdecoder: Processing of the Raw MPRA Data With a priori Unknown Sequences of the Region of Interest and Associated Barcodes

MPRAdecoder: Processing of the Raw MPRA Data With a priori Unknown Sequences of the Region of Interest and Associated Barcodes

Massively parallel reporter assays (MPRAs) enable high-throughput functional evaluation of numerous DNA regulatory elements and/or their mutant variants. The assays are based on the construction of reporter plasmid libraries containing two variable parts, a region of interest (ROI) and a barcode (BC...

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Bibliographic Details
Main Authors: Anna E. Letiagina, Evgeniya S. Omelina, Anton V. Ivankin, Alexey V. Pindyurin
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-05-01
Series:Frontiers in Genetics
Subjects:
massively parallel reporter assay
MPRA
reporter constructs
region of interest
barcodes
next-generation sequencing
Online Access:https://www.frontiersin.org/articles/10.3389/fgene.2021.618189/full

Internet

https://www.frontiersin.org/articles/10.3389/fgene.2021.618189/full

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