Antioxidant Effects and Phytochemical Properties of Seven Taiwanese <i>Cirsium</i> Species Extracts
In the present investigation, we compared the radical-scavenging activities and phenolic contents of seven Taiwanese <i>Cirsium</i> species with a spectrophotometric method. We further analyzed their phytochemical profiles with high-performance liquid chromatography–photodiode array dete...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-06-01
|
Series: | Molecules |
Subjects: | |
Online Access: | https://www.mdpi.com/1420-3049/26/13/3935 |
id |
doaj-6ca322b797e64592b6ac93199ddd5b1d |
---|---|
record_format |
Article |
spelling |
doaj-6ca322b797e64592b6ac93199ddd5b1d2021-07-15T15:42:28ZengMDPI AGMolecules1420-30492021-06-01263935393510.3390/molecules26133935Antioxidant Effects and Phytochemical Properties of Seven Taiwanese <i>Cirsium</i> Species ExtractsZi-Wei Zhao0Hung-Chi Chang1Hui Ching2Jin-Cherng Lien3Hui-Chi Huang4Chi-Rei Wu5The Institute of Basic Medical Sciences, National Cheng Kung University, Tainan 701, TaiwanDepartment of Golden-Ager Industry Management, College of Management, Chaoyang University of Technology, Taichung 413, TaiwanDepartment of Pharmacy, Taichung Hospital, Ministry of Health and Welfare, Taichung 404, TaiwanSchool of Pharmacy, China Medical University, Taichung 404, TaiwanDepartment of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Pharmacy, China Medical University, Taichung 404, TaiwanDepartment of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Pharmacy, China Medical University, Taichung 404, TaiwanIn the present investigation, we compared the radical-scavenging activities and phenolic contents of seven Taiwanese <i>Cirsium</i> species with a spectrophotometric method. We further analyzed their phytochemical profiles with high-performance liquid chromatography–photodiode array detection (HPLC–DAD). We found that the flower part of <i>Cirsium japonicum</i> var. <i>australe</i> (CJF) showed the best radical-scavenging activities against 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and the hypochlorite ion, for which the equivalents were 6.44 ± 0.17 mg catechin/g, 54.85 ± 0.66 mmol Trolox/g and 418.69 ± 10.52 mmol Trolox/g respectively. CJF also had the highest contents of total phenolics (5.23 ± 0.20 mg catechin/g) and phenylpropanoids (29.73 ± 0.72 mg verbascoside/g). According to the Pearson’s correlation coefficient, there was a positive correlation between the total phenylpropanoid content and ABTS radical-scavenging activities (r = 0.979). The radical-scavenging activities of the phenylpropanoids are closely related to their reducing power (r = 0.986). HPLC chromatograms obtained in validated HPLC conditions confirm that they have different phytochemical profiles by which they can be distinguished. Only CJF contained silicristin (0.66 ± 0.03 mg/g) and silydianin (9.13 ± 0.30 mg/g). CJF contained the highest contents of apigenin (5.56 ± 0.09 mg/g) and diosmetin (2.82 ± 0.10 mg/g). Among the major constituents, silicristin had the best radical-scavenging activities against DPPH (71.68 ± 0.66 mg catechin/g) and ABTS (3.01 ± 0.01 mmol Trolox/g). However, diosmetin had the best reducing power and radical-scavenging activity against the hypochlorite anion (41.57 ± 1.14 mg mmol Trolox/g). Finally, we found that flavonolignans (especial silicristin and silydianin) and diosmetin acted synergistically in scavenging radicals.https://www.mdpi.com/1420-3049/26/13/3935<i>Cirsium</i> speciessilymarinsilicristinantioxidant activitiesphytochemical profilesHPLC–DAD |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zi-Wei Zhao Hung-Chi Chang Hui Ching Jin-Cherng Lien Hui-Chi Huang Chi-Rei Wu |
spellingShingle |
Zi-Wei Zhao Hung-Chi Chang Hui Ching Jin-Cherng Lien Hui-Chi Huang Chi-Rei Wu Antioxidant Effects and Phytochemical Properties of Seven Taiwanese <i>Cirsium</i> Species Extracts Molecules <i>Cirsium</i> species silymarin silicristin antioxidant activities phytochemical profiles HPLC–DAD |
author_facet |
Zi-Wei Zhao Hung-Chi Chang Hui Ching Jin-Cherng Lien Hui-Chi Huang Chi-Rei Wu |
author_sort |
Zi-Wei Zhao |
title |
Antioxidant Effects and Phytochemical Properties of Seven Taiwanese <i>Cirsium</i> Species Extracts |
title_short |
Antioxidant Effects and Phytochemical Properties of Seven Taiwanese <i>Cirsium</i> Species Extracts |
title_full |
Antioxidant Effects and Phytochemical Properties of Seven Taiwanese <i>Cirsium</i> Species Extracts |
title_fullStr |
Antioxidant Effects and Phytochemical Properties of Seven Taiwanese <i>Cirsium</i> Species Extracts |
title_full_unstemmed |
Antioxidant Effects and Phytochemical Properties of Seven Taiwanese <i>Cirsium</i> Species Extracts |
title_sort |
antioxidant effects and phytochemical properties of seven taiwanese <i>cirsium</i> species extracts |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2021-06-01 |
description |
In the present investigation, we compared the radical-scavenging activities and phenolic contents of seven Taiwanese <i>Cirsium</i> species with a spectrophotometric method. We further analyzed their phytochemical profiles with high-performance liquid chromatography–photodiode array detection (HPLC–DAD). We found that the flower part of <i>Cirsium japonicum</i> var. <i>australe</i> (CJF) showed the best radical-scavenging activities against 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and the hypochlorite ion, for which the equivalents were 6.44 ± 0.17 mg catechin/g, 54.85 ± 0.66 mmol Trolox/g and 418.69 ± 10.52 mmol Trolox/g respectively. CJF also had the highest contents of total phenolics (5.23 ± 0.20 mg catechin/g) and phenylpropanoids (29.73 ± 0.72 mg verbascoside/g). According to the Pearson’s correlation coefficient, there was a positive correlation between the total phenylpropanoid content and ABTS radical-scavenging activities (r = 0.979). The radical-scavenging activities of the phenylpropanoids are closely related to their reducing power (r = 0.986). HPLC chromatograms obtained in validated HPLC conditions confirm that they have different phytochemical profiles by which they can be distinguished. Only CJF contained silicristin (0.66 ± 0.03 mg/g) and silydianin (9.13 ± 0.30 mg/g). CJF contained the highest contents of apigenin (5.56 ± 0.09 mg/g) and diosmetin (2.82 ± 0.10 mg/g). Among the major constituents, silicristin had the best radical-scavenging activities against DPPH (71.68 ± 0.66 mg catechin/g) and ABTS (3.01 ± 0.01 mmol Trolox/g). However, diosmetin had the best reducing power and radical-scavenging activity against the hypochlorite anion (41.57 ± 1.14 mg mmol Trolox/g). Finally, we found that flavonolignans (especial silicristin and silydianin) and diosmetin acted synergistically in scavenging radicals. |
topic |
<i>Cirsium</i> species silymarin silicristin antioxidant activities phytochemical profiles HPLC–DAD |
url |
https://www.mdpi.com/1420-3049/26/13/3935 |
work_keys_str_mv |
AT ziweizhao antioxidanteffectsandphytochemicalpropertiesofseventaiwaneseicirsiumispeciesextracts AT hungchichang antioxidanteffectsandphytochemicalpropertiesofseventaiwaneseicirsiumispeciesextracts AT huiching antioxidanteffectsandphytochemicalpropertiesofseventaiwaneseicirsiumispeciesextracts AT jinchernglien antioxidanteffectsandphytochemicalpropertiesofseventaiwaneseicirsiumispeciesextracts AT huichihuang antioxidanteffectsandphytochemicalpropertiesofseventaiwaneseicirsiumispeciesextracts AT chireiwu antioxidanteffectsandphytochemicalpropertiesofseventaiwaneseicirsiumispeciesextracts |
_version_ |
1721298750656217088 |