Dietary canolol protects the heart against the deleterious effects induced by the association of rapeseed oil, vitamin E and coenzyme Q10 in the context of a high-fat diet
Abstract Background Obesity progressively leads to cardiac failure. Omega-3 polyunsaturated fatty acids (PUFA) have been shown to have cardio-protective effects in numerous pathological situations. It is not known whether rapeseed oil, which contains α-linolenic acid (ALA), has a similar protective...
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doaj-6cfe691d14ff48149b6a446902e52ea22020-11-24T22:01:25ZengBMCNutrition & Metabolism1743-70752018-02-0115111410.1186/s12986-018-0252-4Dietary canolol protects the heart against the deleterious effects induced by the association of rapeseed oil, vitamin E and coenzyme Q10 in the context of a high-fat dietThibault Leger0Isabelle Hininger-Favier1Frédéric Capel2Alain Geloen3Jean-Paul Rigaudière4Chrystèle Jouve5Elodie Pitois6Gaelle Pineau7Carole Vaysse8Jean-Michel Chardigny9Marie-Caroline Michalski10Corinne Malpuech-Brugère11Luc Demaison12Université Clermont Auvergne, INRA, UNH, Unité de Nutrition Humaine, CRNH AuvergneUniv. Grenoble Alpes, INSERM, LBFAUniversité Clermont Auvergne, INRA, UNH, Unité de Nutrition Humaine, CRNH AuvergneUniv-Lyon, laboratoire CarMeN, INRA UMR1397, INSERM U1060, Université Claude Bernard Lyon 1, INSA-Lyon, IMBLUniversité Clermont Auvergne, INRA, UNH, Unité de Nutrition Humaine, CRNH AuvergneUniversité Clermont Auvergne, INRA, UNH, Unité de Nutrition Humaine, CRNH AuvergneUniversité Clermont Auvergne, INRA, UNH, Unité de Nutrition Humaine, CRNH AuvergneUniv-Lyon, laboratoire CarMeN, INRA UMR1397, INSERM U1060, Université Claude Bernard Lyon 1, INSA-Lyon, IMBLITERG-ENMS, Université de Bordeaux, rue Léo SaignatUniversité Clermont Auvergne, INRA, UNH, Unité de Nutrition Humaine, CRNH AuvergneUniv-Lyon, laboratoire CarMeN, INRA UMR1397, INSERM U1060, Université Claude Bernard Lyon 1, INSA-Lyon, IMBLUniversité Clermont Auvergne, INRA, UNH, Unité de Nutrition Humaine, CRNH AuvergneUniversité Clermont Auvergne, INRA, UNH, Unité de Nutrition Humaine, CRNH AuvergneAbstract Background Obesity progressively leads to cardiac failure. Omega-3 polyunsaturated fatty acids (PUFA) have been shown to have cardio-protective effects in numerous pathological situations. It is not known whether rapeseed oil, which contains α-linolenic acid (ALA), has a similar protective effect. Omega-3 PUFAs are sensitive to attack by reactive oxygen species (ROS), and lipid peroxidation products could damage cardiac cells. We thus tested whether dietary refined rapeseed oil (RSO) associated with or without different antioxidants (vitamin E, coenzyme Q10 and canolol) is cardio-protective in a situation of abdominal obesity. Methods Sixty male Wistar rats were subdivided into 5 groups. Each group was fed a specific diet for 11 weeks: a low-fat diet (3% of lipids, C diet) with compositionally-balanced PUFAs; a high-fat diet rich in palm oil (30% of lipids, PS diet); the PS diet in which 40% of lipids were replaced by RSO (R diet); the R diet supplemented with coenzyme Q10 (CoQ10) and vitamin E (RTC diet); and the RTC diet supplemented with canolol (RTCC diet). At the end of the diet period, the rats were sacrificed and the heart was collected and immediately frozen. Fatty acid composition of cardiac phospholipids was then determined. Several features of cardiac function (fibrosis, inflammation, oxidative stress, apoptosis, metabolism, mitochondrial biogenesis) were also estimated. Results Abdominal obesity reduced cardiac oxidative stress and apoptosis rate by increasing the proportion of arachidonic acid (AA) in membrane phospholipids. Dietary RSO had the same effect, though it normalized the proportion of AA. Adding vitamin E and CoQ10 in the RSO-rich high fat diet had a deleterious effect, increasing fibrosis by increasing angiotensin-2 receptor-1b (Ag2R-1b) mRNA expression. Overexpression of these receptors triggers coronary vasoconstriction, which probably induced ischemia. Canolol supplementation counteracted this deleterious effect by reducing coronary vasoconstriction. Conclusion Canolol was found to counteract the fibrotic effects of vitamin E + CoQ10 on cardiac fibrosis in the context of a high-fat diet enriched with RSO. This effect occurred through a restoration of cardiac Ag2R-1b mRNA expression and decreased ischemia.http://link.springer.com/article/10.1186/s12986-018-0252-4HeartRapeseed oilω3 PUFAsAntioxidantCanololObesity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Thibault Leger Isabelle Hininger-Favier Frédéric Capel Alain Geloen Jean-Paul Rigaudière Chrystèle Jouve Elodie Pitois Gaelle Pineau Carole Vaysse Jean-Michel Chardigny Marie-Caroline Michalski Corinne Malpuech-Brugère Luc Demaison |
spellingShingle |
Thibault Leger Isabelle Hininger-Favier Frédéric Capel Alain Geloen Jean-Paul Rigaudière Chrystèle Jouve Elodie Pitois Gaelle Pineau Carole Vaysse Jean-Michel Chardigny Marie-Caroline Michalski Corinne Malpuech-Brugère Luc Demaison Dietary canolol protects the heart against the deleterious effects induced by the association of rapeseed oil, vitamin E and coenzyme Q10 in the context of a high-fat diet Nutrition & Metabolism Heart Rapeseed oil ω3 PUFAs Antioxidant Canolol Obesity |
author_facet |
Thibault Leger Isabelle Hininger-Favier Frédéric Capel Alain Geloen Jean-Paul Rigaudière Chrystèle Jouve Elodie Pitois Gaelle Pineau Carole Vaysse Jean-Michel Chardigny Marie-Caroline Michalski Corinne Malpuech-Brugère Luc Demaison |
author_sort |
Thibault Leger |
title |
Dietary canolol protects the heart against the deleterious effects induced by the association of rapeseed oil, vitamin E and coenzyme Q10 in the context of a high-fat diet |
title_short |
Dietary canolol protects the heart against the deleterious effects induced by the association of rapeseed oil, vitamin E and coenzyme Q10 in the context of a high-fat diet |
title_full |
Dietary canolol protects the heart against the deleterious effects induced by the association of rapeseed oil, vitamin E and coenzyme Q10 in the context of a high-fat diet |
title_fullStr |
Dietary canolol protects the heart against the deleterious effects induced by the association of rapeseed oil, vitamin E and coenzyme Q10 in the context of a high-fat diet |
title_full_unstemmed |
Dietary canolol protects the heart against the deleterious effects induced by the association of rapeseed oil, vitamin E and coenzyme Q10 in the context of a high-fat diet |
title_sort |
dietary canolol protects the heart against the deleterious effects induced by the association of rapeseed oil, vitamin e and coenzyme q10 in the context of a high-fat diet |
publisher |
BMC |
series |
Nutrition & Metabolism |
issn |
1743-7075 |
publishDate |
2018-02-01 |
description |
Abstract Background Obesity progressively leads to cardiac failure. Omega-3 polyunsaturated fatty acids (PUFA) have been shown to have cardio-protective effects in numerous pathological situations. It is not known whether rapeseed oil, which contains α-linolenic acid (ALA), has a similar protective effect. Omega-3 PUFAs are sensitive to attack by reactive oxygen species (ROS), and lipid peroxidation products could damage cardiac cells. We thus tested whether dietary refined rapeseed oil (RSO) associated with or without different antioxidants (vitamin E, coenzyme Q10 and canolol) is cardio-protective in a situation of abdominal obesity. Methods Sixty male Wistar rats were subdivided into 5 groups. Each group was fed a specific diet for 11 weeks: a low-fat diet (3% of lipids, C diet) with compositionally-balanced PUFAs; a high-fat diet rich in palm oil (30% of lipids, PS diet); the PS diet in which 40% of lipids were replaced by RSO (R diet); the R diet supplemented with coenzyme Q10 (CoQ10) and vitamin E (RTC diet); and the RTC diet supplemented with canolol (RTCC diet). At the end of the diet period, the rats were sacrificed and the heart was collected and immediately frozen. Fatty acid composition of cardiac phospholipids was then determined. Several features of cardiac function (fibrosis, inflammation, oxidative stress, apoptosis, metabolism, mitochondrial biogenesis) were also estimated. Results Abdominal obesity reduced cardiac oxidative stress and apoptosis rate by increasing the proportion of arachidonic acid (AA) in membrane phospholipids. Dietary RSO had the same effect, though it normalized the proportion of AA. Adding vitamin E and CoQ10 in the RSO-rich high fat diet had a deleterious effect, increasing fibrosis by increasing angiotensin-2 receptor-1b (Ag2R-1b) mRNA expression. Overexpression of these receptors triggers coronary vasoconstriction, which probably induced ischemia. Canolol supplementation counteracted this deleterious effect by reducing coronary vasoconstriction. Conclusion Canolol was found to counteract the fibrotic effects of vitamin E + CoQ10 on cardiac fibrosis in the context of a high-fat diet enriched with RSO. This effect occurred through a restoration of cardiac Ag2R-1b mRNA expression and decreased ischemia. |
topic |
Heart Rapeseed oil ω3 PUFAs Antioxidant Canolol Obesity |
url |
http://link.springer.com/article/10.1186/s12986-018-0252-4 |
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