Establishment of two-dimensional gel electrophoresis for soybean protein isolate and its application

To optimize the conditions for the establishment of two-dimensional gel electrophoresis (2-DE) of soybean protein isolate (SPI), we investigated Ampholine mixture, anodic and cathodic electrolytes, loading amount of sample, acrylamide concentration, pH gradient and gel staining method in two-dimensi...

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Bibliographic Details
Main Authors: Xinkai Lu, Yaoming Cui, Junjun Guan, Xue Liu, Hao Zhu, Xuyang Ji, Jianzhang Zheng, Yunlong Cheng, Xiaofei Fu
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2020-09-01
Series:Grain & Oil Science and Technology
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2590259820300054