Real-time tracking of complex ubiquitination cascades using a fluorescent confocal on-bead assay

Real-time tracking of complex ubiquitination cascades using a fluorescent confocal on-bead assay

Abstract Background The ubiquitin-proteasome system (UPS) controls the stability, localization and/or activity of the proteome. However, the identification and characterization of complex individual ubiquitination cascades and their modulators remains a challenge. Here, we report a broadly applicabl...

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Bibliographic Details
Main Authors: Joanna Koszela, Nhan T. Pham, David Evans, Stefan Mann, Irene Perez-Pi, Steven Shave, Derek F. J. Ceccarelli, Frank Sicheri, Mike Tyers, Manfred Auer
Format: Article
Language:English
Published: BMC 2018-08-01
Series:BMC Biology
Subjects:
Ubiquitin
Inhibitors
Confocal fluorescence
Bead-based assay
Ubiquitination assay
Online Access:http://link.springer.com/article/10.1186/s12915-018-0554-z

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http://link.springer.com/article/10.1186/s12915-018-0554-z

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