Influence of ascorbic acid and α-tocopherol on the autoxidation and in vitro antifungal activity of amphotericin B
Background and Purpose: Amphotericin B (AmB) is the standard treatment for systemic fungal infections; however, the formation of reactive oxygen species reduces the efficacy and stability of this molecule. The present study aimed to evaluate the effect of the combination of AmB with ascorbic acid an...
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Mazandaran University of Medical Sciences
2021-03-01
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doaj-6d4d27b57c2645a392918a28f402b80b2021-07-14T06:25:20ZengMazandaran University of Medical SciencesCurrent Medical Mycology2423-34392423-34202021-03-0171121810.18502/cmm.7.1.6178127275Influence of ascorbic acid and α-tocopherol on the autoxidation and in vitro antifungal activity of amphotericin BMohammed Habib Belhachemi0Zahia Boucherit-Otmani1Kebir Boucherit2Sara Belmir3Department of Biology, Faculty of Natural Sciences and Life Sciences and Earth and the Universe, University of Tlemcen, AlgeriaDepartment of Biology, Faculty of Natural Sciences and Life Sciences and Earth and the Universe, University of Tlemcen, AlgeriaDepartment of Biology, Faculty of Natural Sciences and Life Sciences and Earth and the Universe, University of Tlemcen, AlgeriaDepartment of Biology, Faculty of Natural Sciences and Life Sciences and Earth and the Universe, University of Tlemcen, AlgeriaBackground and Purpose: Amphotericin B (AmB) is the standard treatment for systemic fungal infections; however, the formation of reactive oxygen species reduces the efficacy and stability of this molecule. The present study aimed to evaluate the effect of the combination of AmB with ascorbic acid and α-tocopherol on its autoxidation and antifungal activity. Materials and Methods: The antifungal activity against Candida albicans was evaluated by the viable cell counting method and checking their morphological changes with a scanning electron microscope. Monomer state of AmB was assessed by scanning the UV absorbance in the range of 300-450 nm and the lipid peroxidation was measured using quantification of thiobarbituric acid reactive-substances (TBARS). Results: Based on the findings, the addition of ascorbic acid (3×102 µg/mL) and α-tocopherol (16 µg/mL) to the reaction medium of AmB increased its antifungal activity while maintaining its molecular stability. Moreover, the level of TBARS formed in the reaction medium of AmB was significantly reduced after combination with ascorbic acid and α-tocopherol. Conclusion: Given their availability, their anti-free radical activity, and their low toxicity, the incorporation of ascorbic acid and α-tocopherol into the reaction medium of AmB seems to be a promising approach to obtain an effective antifungal formulation.http://cmm.mazums.ac.ir/article_127275_1df14b131413cf2f58f6bb6e42b57c71.pdfamphotericin bantifungal activityascorbic acidα-tocopherolautoxidation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mohammed Habib Belhachemi Zahia Boucherit-Otmani Kebir Boucherit Sara Belmir |
spellingShingle |
Mohammed Habib Belhachemi Zahia Boucherit-Otmani Kebir Boucherit Sara Belmir Influence of ascorbic acid and α-tocopherol on the autoxidation and in vitro antifungal activity of amphotericin B Current Medical Mycology amphotericin b antifungal activity ascorbic acid α-tocopherol autoxidation |
author_facet |
Mohammed Habib Belhachemi Zahia Boucherit-Otmani Kebir Boucherit Sara Belmir |
author_sort |
Mohammed Habib Belhachemi |
title |
Influence of ascorbic acid and α-tocopherol on the autoxidation and in vitro antifungal activity of amphotericin B |
title_short |
Influence of ascorbic acid and α-tocopherol on the autoxidation and in vitro antifungal activity of amphotericin B |
title_full |
Influence of ascorbic acid and α-tocopherol on the autoxidation and in vitro antifungal activity of amphotericin B |
title_fullStr |
Influence of ascorbic acid and α-tocopherol on the autoxidation and in vitro antifungal activity of amphotericin B |
title_full_unstemmed |
Influence of ascorbic acid and α-tocopherol on the autoxidation and in vitro antifungal activity of amphotericin B |
title_sort |
influence of ascorbic acid and α-tocopherol on the autoxidation and in vitro antifungal activity of amphotericin b |
publisher |
Mazandaran University of Medical Sciences |
series |
Current Medical Mycology |
issn |
2423-3439 2423-3420 |
publishDate |
2021-03-01 |
description |
Background and Purpose: Amphotericin B (AmB) is the standard treatment for systemic fungal infections; however, the formation of reactive oxygen species reduces the efficacy and stability of this molecule. The present study aimed to evaluate the effect of the combination of AmB with ascorbic acid and α-tocopherol on its autoxidation and antifungal activity. Materials and Methods: The antifungal activity against Candida albicans was evaluated by the viable cell counting method and checking their morphological changes with a scanning electron microscope. Monomer state of AmB was assessed by scanning the UV absorbance in the range of 300-450 nm and the lipid peroxidation was measured using quantification of thiobarbituric acid reactive-substances (TBARS). Results: Based on the findings, the addition of ascorbic acid (3×102 µg/mL) and α-tocopherol (16 µg/mL) to the reaction medium of AmB increased its antifungal activity while maintaining its molecular stability. Moreover, the level of TBARS formed in the reaction medium of AmB was significantly reduced after combination with ascorbic acid and α-tocopherol. Conclusion: Given their availability, their anti-free radical activity, and their low toxicity, the incorporation of ascorbic acid and α-tocopherol into the reaction medium of AmB seems to be a promising approach to obtain an effective antifungal formulation. |
topic |
amphotericin b antifungal activity ascorbic acid α-tocopherol autoxidation |
url |
http://cmm.mazums.ac.ir/article_127275_1df14b131413cf2f58f6bb6e42b57c71.pdf |
work_keys_str_mv |
AT mohammedhabibbelhachemi influenceofascorbicacidandatocopherolontheautoxidationandinvitroantifungalactivityofamphotericinb AT zahiaboucheritotmani influenceofascorbicacidandatocopherolontheautoxidationandinvitroantifungalactivityofamphotericinb AT kebirboucherit influenceofascorbicacidandatocopherolontheautoxidationandinvitroantifungalactivityofamphotericinb AT sarabelmir influenceofascorbicacidandatocopherolontheautoxidationandinvitroantifungalactivityofamphotericinb |
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