In vitro cultivation of rat bone marrow mesenchymal stem cells and establishment of pEGFP/Ang-1 transfection method

Objective: To obtain the bone marrow mesenchymal stem cells (BMSCs), complete phenotypic identification and successfully transfect rat BMSCs by recombinant plasmid pEGFP/Ang-1. Methods: BMSCs were isolated from bone marrow using density gradient centrifugation method and adherence screening method,...

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Bibliographic Details
Main Authors: Xiu-Qun Zhang, Long Wang, Shu-Li Zhao, Wei Xu
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2014-09-01
Series:Asian Pacific Journal of Tropical Biomedicine
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2221169115300915