In vitro cultivation of rat bone marrow mesenchymal stem cells and establishment of pEGFP/Ang-1 transfection method
Objective: To obtain the bone marrow mesenchymal stem cells (BMSCs), complete phenotypic identification and successfully transfect rat BMSCs by recombinant plasmid pEGFP/Ang-1. Methods: BMSCs were isolated from bone marrow using density gradient centrifugation method and adherence screening method,...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
Wolters Kluwer Medknow Publications
2014-09-01
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Series: | Asian Pacific Journal of Tropical Biomedicine |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2221169115300915 |