Organization and ELISA-Based Results of the First Proficiency Testing to Evaluate the Ability of European Union Laboratories to Detect Staphylococcal Enterotoxin Type B (SEB) in Buffer and Milk

Organization and ELISA-Based Results of the First Proficiency Testing to Evaluate the Ability of European Union Laboratories to Detect Staphylococcal Enterotoxin Type B (SEB) in Buffer and Milk

The aim of this work was to organize the first proficiency test (PT) dedicated to staphylococcal enterotoxin B (SEB) detection in milk and buffer solutions. This paper describes the organization of the PT trial according to EN ISO 17043 requirements. Characterization of the SEB stock solution was pe...

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Main Authors: Yacine Nia, Mélanie Rodriguez, Reinhard Zeleny, Sabine Herbin, Frédéric Auvray, Uwe Fiebig, Marc-André Avondet, Amalia Munoz, Jacques-Antoine Hennekinne
Format: Article
Language:English
Published: MDPI AG 2016-09-01
Series:Toxins
Subjects:
staphylococcal enterotoxin B
bioterrorism agent
proficiency test
ELISA methods
Online Access:http://www.mdpi.com/2072-6651/8/9/268
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spelling doaj-6d7ef0ef85d44cd1b90efbb13dc749ae2020-11-25T00:02:15ZengMDPI AGToxins2072-66512016-09-018926810.3390/toxins8090268toxins8090268Organization and ELISA-Based Results of the First Proficiency Testing to Evaluate the Ability of European Union Laboratories to Detect Staphylococcal Enterotoxin Type B (SEB) in Buffer and MilkYacine Nia0Mélanie Rodriguez1Reinhard Zeleny2Sabine Herbin3Frédéric Auvray4Uwe Fiebig5Marc-André Avondet6Amalia Munoz7Jacques-Antoine Hennekinne8Laboratory for food safety, ANSES, Université Paris-Est, Maisons-Alfort F-94700, FranceLaboratory for food safety, ANSES, Université Paris-Est, Maisons-Alfort F-94700, FranceJoint Research Centre, Directorate for Health, Consumers and Reference Materials, European Commission, Retieseweg 111, Geel 2440, BelgiumLaboratory for food safety, ANSES, Université Paris-Est, Maisons-Alfort F-94700, FranceLaboratory for food safety, ANSES, Université Paris-Est, Maisons-Alfort F-94700, FranceCentre for Biological Threats and Special Pathogens Biological Toxins, Robert Koch Institute, Berlin 13353, GermanyLABOR SPIEZ, Eidgenössisches Departement für Verteidigung, Bevölkerungsschutz und Sport VBS Bundesamt für Bevölkerungsschutz BABS, Spiez 3700, SwitzerlandJoint Research Centre, Directorate for Health, Consumers and Reference Materials, European Commission, Retieseweg 111, Geel 2440, BelgiumLaboratory for food safety, ANSES, Université Paris-Est, Maisons-Alfort F-94700, FranceThe aim of this work was to organize the first proficiency test (PT) dedicated to staphylococcal enterotoxin B (SEB) detection in milk and buffer solutions. This paper describes the organization of the PT trial according to EN ISO 17043 requirements. Characterization of the SEB stock solution was performed using SDS-PAGE and SE-specific ELISA, and amino acid analysis was used to assign its protein concentration. The solution was then used to prepare six PT materials (four milk and two buffer batches) at a ng/g toxin level, which included one blank and one SEA-containing milk as specificity control. Suitable material homogeneity and stability were assessed using screening and quantitative ELISAs. Among the methods used by the participants, ELISA-based methods demonstrated their efficiency for the detection of SEB in both simple and complex matrices. The results serve as a basis for further improving the detection capabilities in expert laboratories and can therefore be considered as a contribution to biopreparedness.http://www.mdpi.com/2072-6651/8/9/268staphylococcal enterotoxin Bbioterrorism agentproficiency testELISA methods
collection DOAJ
language English
format Article
sources DOAJ
author Yacine Nia
Mélanie Rodriguez
Reinhard Zeleny
Sabine Herbin
Frédéric Auvray
Uwe Fiebig
Marc-André Avondet
Amalia Munoz
Jacques-Antoine Hennekinne
spellingShingle Yacine Nia
Mélanie Rodriguez
Reinhard Zeleny
Sabine Herbin
Frédéric Auvray
Uwe Fiebig
Marc-André Avondet
Amalia Munoz
Jacques-Antoine Hennekinne
Organization and ELISA-Based Results of the First Proficiency Testing to Evaluate the Ability of European Union Laboratories to Detect Staphylococcal Enterotoxin Type B (SEB) in Buffer and Milk
Toxins
staphylococcal enterotoxin B
bioterrorism agent
proficiency test
ELISA methods
author_facet Yacine Nia
Mélanie Rodriguez
Reinhard Zeleny
Sabine Herbin
Frédéric Auvray
Uwe Fiebig
Marc-André Avondet
Amalia Munoz
Jacques-Antoine Hennekinne
author_sort Yacine Nia
title Organization and ELISA-Based Results of the First Proficiency Testing to Evaluate the Ability of European Union Laboratories to Detect Staphylococcal Enterotoxin Type B (SEB) in Buffer and Milk
title_short Organization and ELISA-Based Results of the First Proficiency Testing to Evaluate the Ability of European Union Laboratories to Detect Staphylococcal Enterotoxin Type B (SEB) in Buffer and Milk
title_full Organization and ELISA-Based Results of the First Proficiency Testing to Evaluate the Ability of European Union Laboratories to Detect Staphylococcal Enterotoxin Type B (SEB) in Buffer and Milk
title_fullStr Organization and ELISA-Based Results of the First Proficiency Testing to Evaluate the Ability of European Union Laboratories to Detect Staphylococcal Enterotoxin Type B (SEB) in Buffer and Milk
title_full_unstemmed Organization and ELISA-Based Results of the First Proficiency Testing to Evaluate the Ability of European Union Laboratories to Detect Staphylococcal Enterotoxin Type B (SEB) in Buffer and Milk
title_sort organization and elisa-based results of the first proficiency testing to evaluate the ability of european union laboratories to detect staphylococcal enterotoxin type b (seb) in buffer and milk
publisher MDPI AG
series Toxins
issn 2072-6651
publishDate 2016-09-01
description The aim of this work was to organize the first proficiency test (PT) dedicated to staphylococcal enterotoxin B (SEB) detection in milk and buffer solutions. This paper describes the organization of the PT trial according to EN ISO 17043 requirements. Characterization of the SEB stock solution was performed using SDS-PAGE and SE-specific ELISA, and amino acid analysis was used to assign its protein concentration. The solution was then used to prepare six PT materials (four milk and two buffer batches) at a ng/g toxin level, which included one blank and one SEA-containing milk as specificity control. Suitable material homogeneity and stability were assessed using screening and quantitative ELISAs. Among the methods used by the participants, ELISA-based methods demonstrated their efficiency for the detection of SEB in both simple and complex matrices. The results serve as a basis for further improving the detection capabilities in expert laboratories and can therefore be considered as a contribution to biopreparedness.
topic staphylococcal enterotoxin B
bioterrorism agent
proficiency test
ELISA methods
url http://www.mdpi.com/2072-6651/8/9/268
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