Application of Real-time PCR for Rapid Petection of Salmonella spp., Salmonella enterica ser. Typhimurium and Enteritidis in Food Samples of Animal Origin without Pre-enrichment and with Pre-enrichment

Application of Real-time PCR for Rapid Petection of Salmonella spp., Salmonella enterica ser. Typhimurium and Enteritidis in Food Samples of Animal Origin without Pre-enrichment and with Pre-enrichment

The aim of this study was follow the contamination of milk and meat products with Salmonella spp. by using the Step One real-time PCR. We used the PrepSEQ Rapid Spin Sample Preparation Kit for isolation of DNA and Power SYBR Green PCR Master Mix for pursuance the real-time PCR (Applied Biosystems)....

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Bibliographic Details
Main Authors: Jaroslav Pochop, Miroslava Kačániová, Lukáš Hleba, Ľubomír Lopašovský, Katarína Rovná, Henrieta Arpášová
Format: Article
Language:English
Published: Agroprint Timisoara 2012-05-01
Series:Scientific Papers Animal Science and Biotechnologies
Subjects:
pre-enrichment
real-time pcr
salmonella spp.
sybr green
Online Access:http://spasb.ro/index.php/spasb/article/view/596

Internet

http://spasb.ro/index.php/spasb/article/view/596

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