Block Face Scanning Electron Microscopy of Fluorescently Labeled Axons Without Using Near Infra-Red Branding

Block Face Scanning Electron Microscopy of Fluorescently Labeled Axons Without Using Near Infra-Red Branding

In this article, we describe the method that allows fluorescently tagged structures such as axons to be targeted for electron microscopy (EM) analysis without the need to convert their labels into electron dense stains, introduce any fiducial marks, or image large volumes at high resolution. We opti...

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Bibliographic Details
Main Authors: Catherine Maclachlan, Daniela A. Sahlender, Shuichi Hayashi, Zoltán Molnár, Graham Knott
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-11-01
Series:Frontiers in Neuroanatomy
Subjects:
correlative light and electron microscopy (CLEM)
axons
scanning electron microscopy
neuron ultrastructure
serial block-face electron microscopy (SBEM)
Online Access:https://www.frontiersin.org/article/10.3389/fnana.2018.00088/full

Internet

https://www.frontiersin.org/article/10.3389/fnana.2018.00088/full

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