A method for high-throughput screening hydrolase of lignin β-aryl ether linkage from directed evolution by glutathione (GSH) assay
Abstract Background Lignin’s valorization plays a critical role in refining the bioresource. Considering that the β-aryl ether linkage (β-O-4 bond) accounts for 50–70% of lignin chemical linkage between aromatic rings, the hydrolase of lignin β-aryl ether linkage, especially the β-etherase, provided...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
SpringerOpen
2020-05-01
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Series: | Bioresources and Bioprocessing |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s40643-020-00317-7 |