A method for high-throughput screening hydrolase of lignin β-aryl ether linkage from directed evolution by glutathione (GSH) assay

A method for high-throughput screening hydrolase of lignin β-aryl ether linkage from directed evolution by glutathione (GSH) assay

Abstract Background Lignin’s valorization plays a critical role in refining the bioresource. Considering that the β-aryl ether linkage (β-O-4 bond) accounts for 50–70% of lignin chemical linkage between aromatic rings, the hydrolase of lignin β-aryl ether linkage, especially the β-etherase, provided...

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Bibliographic Details
Main Authors: Jiaxi Wang, Kun Yan, Wenya Wang, Yan Zhou
Format: Article
Language:English
Published: SpringerOpen 2020-05-01
Series:Bioresources and Bioprocessing
Subjects:
Lignin valorization
β-etherase
Directed evolution
High-throughput screening
Online Access:http://link.springer.com/article/10.1186/s40643-020-00317-7

Internet

http://link.springer.com/article/10.1186/s40643-020-00317-7

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