Structures of HIV-1 Neutralizing Antibody 10E8 Delineate the Mechanistic Basis of Its Multi-Peak Behavior on Size-Exclusion Chromatography

Structures of HIV-1 Neutralizing Antibody 10E8 Delineate the Mechanistic Basis of Its Multi-Peak Behavior on Size-Exclusion Chromatography

Antibody 10E8 is capable of effectively neutralizing HIV through its recognition of the membrane-proximal external region (MPER), and a suitably optimized version of 10E8 might have utility in HIV therapy and prophylaxis. However, 10E8 displays a three-peak profile on size-exclusion chromatography (...

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Bibliographic Details
Main Authors: Young Do Kwon, Xiangchun E. Wang, Michael F. Bender, Rong Yang, Yile Li, Krisha McKee, Reda Rawi, Sijy O’Dell, Nicole A. Schneck, Andrew Shaddeau, Baoshan Zhang, Frank J. Arnold, Mark Connors, Nicole A. Doria-Rose, Peter D. Kwong, Q. Paula Lei
Format: Article
Language:English
Published: MDPI AG 2021-06-01
Series:Antibodies
Subjects:
cis-trans proline isomerization
conformational isomerization
hydrophobic interaction chromatography (HIC)
mobile phase modifier
monoclonal antibody 10E8
MPER
Online Access:https://www.mdpi.com/2073-4468/10/2/23

Internet

https://www.mdpi.com/2073-4468/10/2/23

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