BAMClipper: removing primers from alignments to minimize false-negative mutations in amplicon next-generation sequencing

BAMClipper: removing primers from alignments to minimize false-negative mutations in amplicon next-generation sequencing

Abstract Amplicon-based next-generation sequencing (NGS) has been widely adopted for genetic variation detection in human and other organisms. Conventional data analysis paradigm includes primer trimming before read mapping. Here we introduce BAMClipper that removes primer sequences after mapping or...

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Bibliographic Details
Main Authors: Chun Hang Au, Dona N. Ho, Ava Kwong, Tsun Leung Chan, Edmond S. K. Ma
Format: Article
Language:English
Published: Nature Publishing Group 2017-05-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-017-01703-6

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https://doi.org/10.1038/s41598-017-01703-6

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