Application of CRISPR/Cas9 editing and digital droplet PCR in human iPSCs to generate novel knock-in reporter lines to visualize dopaminergic neurons

Application of CRISPR/Cas9 editing and digital droplet PCR in human iPSCs to generate novel knock-in reporter lines to visualize dopaminergic neurons

Human induced pluripotent stem cells (hiPSCs) have become indispensable for disease modelling. They are an important resource to access patient cells harbouring disease-causing mutations. Derivation of midbrain dopaminergic (DAergic) neurons from hiPSCs of PD patients represents the only option to m...

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Bibliographic Details
Main Authors: Christa Überbacher, Julia Obergasteiger, Mattia Volta, Serena Venezia, Stefan Müller, Isabella Pesce, Sara Pizzi, Giulia Lamonaca, Anne Picard, Giada Cattelan, Giorgio Malpeli, Michele Zoli, Dayne Beccano-Kelly, Rowan Flynn, Richard Wade-Martins, Peter P. Pramstaller, Andrew A. Hicks, Sally A. Cowley, Corrado Corti
Format: Article
Language:English
Published: Elsevier 2019-12-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506119302867

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http://www.sciencedirect.com/science/article/pii/S1873506119302867

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