Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair

Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair

Altering cellular responses to double-strand breaks in DNA could rebalance CRISPRediting outcomes. Here, the authors use a pooled CRISPR screen to identify inhibition of CDC7 as a strategy to improve HDR outcomes.

Bibliographic Details
Main Authors: Beeke Wienert, David N. Nguyen, Alexis Guenther, Sharon J. Feng, Melissa N. Locke, Stacia K. Wyman, Jiyung Shin, Katelynn R. Kazane, Georgia L. Gregory, Matthew A. M. Carter, Francis Wright, Bruce R. Conklin, Alex Marson, Chris D. Richardson, Jacob E. Corn
Format: Article
Language:English
Published: Nature Publishing Group 2020-04-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-15845-1

Internet

https://doi.org/10.1038/s41467-020-15845-1

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