Improved library preparation protocols for amplicon sequencing-based noninvasive fetal genotyping for RHD-positive D antigen-negative alleles

Improved library preparation protocols for amplicon sequencing-based noninvasive fetal genotyping for RHD-positive D antigen-negative alleles

Abstract Objective We aimed to simplify our fetal RHD genotyping protocol by changing the method to attach Illumina’s sequencing adaptors to PCR products from the ligation-based method to a PCR-based method, and to improve its reliability and robustness by introducing unique molecular indexes, which...

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Bibliographic Details
Main Authors: Asuka Hori, Hiroko Ogata-Kawata, Aiko Sasaki, Ken Takahashi, Kosuke Taniguchi, Ohsuke Migita, Akihiro Kawashima, Aikou Okamoto, Akihiko Sekizawa, Haruhiko Sago, Fumio Takada, Kazuhiko Nakabayashi, Kenichiro Hata
Format: Article
Language:English
Published: BMC 2021-09-01
Series:BMC Research Notes
Subjects:
RHD
Cell-free DNA (cfDNA)
Non-invasive prenatal testing (NIPT)
Amplicon sequencing
Unique molecular identifier (UMI)
Online Access:https://doi.org/10.1186/s13104-021-05793-4

Internet

https://doi.org/10.1186/s13104-021-05793-4

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