Genotype-independent Agrobacterium rhizogenes-mediated root transformation of chickpea: a rapid and efficient method for reverse genetics studies

Genotype-independent Agrobacterium rhizogenes-mediated root transformation of chickpea: a rapid and efficient method for reverse genetics studies

Abstract Background Chickpea (Cicer arietinum L.), an important legume crop is one of the major source of dietary protein. Developing an efficient and reproducible transformation method is imperative to expedite functional genomics studies in this crop. Here, we present an optimized and detailed pro...

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Bibliographic Details
Main Authors: Pooja Rani Aggarwal, Papri Nag, Pooja Choudhary, Niranjan Chakraborty, Subhra Chakraborty
Format: Article
Language:English
Published: BMC 2018-07-01
Series:Plant Methods
Subjects:
Legumes
Cicer arietinum
Agrobacterium rhizogenes, strain K599
Transformation efficiency
Functional genomics
Green fluorescent protein (GFP) expression
Online Access:http://link.springer.com/article/10.1186/s13007-018-0315-6

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http://link.springer.com/article/10.1186/s13007-018-0315-6

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