Use of a bioinformatic-assisted primer design strategy to establish a new nested PCR-based method for Cryptosporidium

Use of a bioinformatic-assisted primer design strategy to establish a new nested PCR-based method for Cryptosporidium

Abstract Background The accurate tracking of Cryptosporidium in faecal, water and/or soil samples in water catchment areas is central to developing strategies to manage the potential risk of cryptosporidiosis transmission to humans. Various PCR assays are used for this purpose. Although some assays...

Full description

Bibliographic Details
Main Authors: Anson V. Koehler, Pasi K. Korhonen, Ross S. Hall, Neil D. Young, Tao Wang, Shane R. Haydon, Robin B. Gasser
Format: Article
Language:English
Published: BMC 2017-10-01
Series:Parasites & Vectors
Subjects:
Cryptosporidium
Bioinformatics
Large subunit of nuclear ribosomal RNA gene (LSU)
D8 domain
Primers
Polymerase chain reaction (PCR)
Online Access:http://link.springer.com/article/10.1186/s13071-017-2462-4

Internet

http://link.springer.com/article/10.1186/s13071-017-2462-4

Similar Items