How Supraphysiological Oxygen Levels in Standard Cell Culture Affect Oxygen-Consuming Reactions

Most mammalian tissue cells experience oxygen partial pressures in vivo equivalent to 1–6% O2 (i.e., physioxia). In standard cell culture, however, headspace O2 levels are usually not actively regulated and under these conditions are ~18%. This drives hyperoxia in cell culture media that can affect...

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Bibliographic Details
Main Authors: Jeffrey A. Stuart, Joao Fonseca, Fereshteh Moradi, Cassandra Cunningham, Bishoy Seliman, Cydney R. Worsfold, Sarah Dolan, John Abando, Lucas A. Maddalena
Format: Article
Language:English
Published: Hindawi Limited 2018-01-01
Series:Oxidative Medicine and Cellular Longevity
Online Access:http://dx.doi.org/10.1155/2018/8238459