Global profiling in vestibular schwannomas shows critical deregulation of microRNAs and upregulation in those included in chromosomal region 14q32.
BACKGROUND: Vestibular schwannomas are benign tumors that arise from Schwann cells in the VIII cranial pair and usually present NF2 gene mutations and/or loss of heterozygosity on chromosome 22q. Deregulation has also been found in several genes, such as ERBB2 and NRG1. MicroRNAs are non-coding RNAs...
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doaj-6f66e52d5e954454837d9f4113979c8b2020-11-25T01:53:42ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0186e6586810.1371/journal.pone.0065868Global profiling in vestibular schwannomas shows critical deregulation of microRNAs and upregulation in those included in chromosomal region 14q32.Miguel Torres-MartinLuis LassalettaJose M de CamposAlberto IslaJavier GavilanGiovanny R PintoRommel R BurbanoFarida LatifBarbara MelendezJavier S CastresanaJuan A ReyBACKGROUND: Vestibular schwannomas are benign tumors that arise from Schwann cells in the VIII cranial pair and usually present NF2 gene mutations and/or loss of heterozygosity on chromosome 22q. Deregulation has also been found in several genes, such as ERBB2 and NRG1. MicroRNAs are non-coding RNAs approximately 21 to 23 nucleotides in length that regulate mRNAs, usually by degradation at the post-transcriptional level. METHODS: We used microarray technology to test the deregulation of miRNAs and other non-coding RNAs present in GeneChip miRNA 1.0 (Affymetrix) over 16 vestibular schwannomas and 3 control-nerves, validating 10 of them by qRT-PCR. FINDINGS: Our results showed the deregulation of 174 miRNAs, including miR-10b, miR-206, miR-183 and miR-204, and the upregulation of miR-431, miR-221, miR-21 and miR-720, among others. The results also showed an aberrant expression of other non-coding RNAs. We also found a general upregulation of the miRNA cluster located at chromosome 14q32. CONCLUSION: Our results suggest that several miRNAs are involved in tumor formation and/or maintenance and that global upregulation of the 14q32 chromosomal site contains miRNAs that may represent a therapeutic target for this neoplasm.http://europepmc.org/articles/PMC3679163?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Miguel Torres-Martin Luis Lassaletta Jose M de Campos Alberto Isla Javier Gavilan Giovanny R Pinto Rommel R Burbano Farida Latif Barbara Melendez Javier S Castresana Juan A Rey |
spellingShingle |
Miguel Torres-Martin Luis Lassaletta Jose M de Campos Alberto Isla Javier Gavilan Giovanny R Pinto Rommel R Burbano Farida Latif Barbara Melendez Javier S Castresana Juan A Rey Global profiling in vestibular schwannomas shows critical deregulation of microRNAs and upregulation in those included in chromosomal region 14q32. PLoS ONE |
author_facet |
Miguel Torres-Martin Luis Lassaletta Jose M de Campos Alberto Isla Javier Gavilan Giovanny R Pinto Rommel R Burbano Farida Latif Barbara Melendez Javier S Castresana Juan A Rey |
author_sort |
Miguel Torres-Martin |
title |
Global profiling in vestibular schwannomas shows critical deregulation of microRNAs and upregulation in those included in chromosomal region 14q32. |
title_short |
Global profiling in vestibular schwannomas shows critical deregulation of microRNAs and upregulation in those included in chromosomal region 14q32. |
title_full |
Global profiling in vestibular schwannomas shows critical deregulation of microRNAs and upregulation in those included in chromosomal region 14q32. |
title_fullStr |
Global profiling in vestibular schwannomas shows critical deregulation of microRNAs and upregulation in those included in chromosomal region 14q32. |
title_full_unstemmed |
Global profiling in vestibular schwannomas shows critical deregulation of microRNAs and upregulation in those included in chromosomal region 14q32. |
title_sort |
global profiling in vestibular schwannomas shows critical deregulation of micrornas and upregulation in those included in chromosomal region 14q32. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2013-01-01 |
description |
BACKGROUND: Vestibular schwannomas are benign tumors that arise from Schwann cells in the VIII cranial pair and usually present NF2 gene mutations and/or loss of heterozygosity on chromosome 22q. Deregulation has also been found in several genes, such as ERBB2 and NRG1. MicroRNAs are non-coding RNAs approximately 21 to 23 nucleotides in length that regulate mRNAs, usually by degradation at the post-transcriptional level. METHODS: We used microarray technology to test the deregulation of miRNAs and other non-coding RNAs present in GeneChip miRNA 1.0 (Affymetrix) over 16 vestibular schwannomas and 3 control-nerves, validating 10 of them by qRT-PCR. FINDINGS: Our results showed the deregulation of 174 miRNAs, including miR-10b, miR-206, miR-183 and miR-204, and the upregulation of miR-431, miR-221, miR-21 and miR-720, among others. The results also showed an aberrant expression of other non-coding RNAs. We also found a general upregulation of the miRNA cluster located at chromosome 14q32. CONCLUSION: Our results suggest that several miRNAs are involved in tumor formation and/or maintenance and that global upregulation of the 14q32 chromosomal site contains miRNAs that may represent a therapeutic target for this neoplasm. |
url |
http://europepmc.org/articles/PMC3679163?pdf=render |
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