Evaluation and Adaptation of a Laboratory-Based cDNA Library Preparation Protocol for Retrospective Sequencing of Archived MicroRNAs from up to 35-Year-Old Clinical FFPE Specimens

Evaluation and Adaptation of a Laboratory-Based cDNA Library Preparation Protocol for Retrospective Sequencing of Archived MicroRNAs from up to 35-Year-Old Clinical FFPE Specimens

Formalin-fixed paraffin-embedded (FFPE) specimens, when used in conjunction with patient clinical data history, represent an invaluable resource for molecular studies of cancer. Even though nucleic acids extracted from archived FFPE tissues are degraded, their molecular analysis has become possible....

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Bibliographic Details
Main Authors: Olivier Loudig, Tao Wang, Kenny Ye, Juan Lin, Yihong Wang, Andrew Ramnauth, Christina Liu, Azadeh Stark, Dhananjay Chitale, Robert Greenlee, Deborah Multerer, Stacey Honda, Yihe Daida, Heather Spencer Feigelson, Andrew Glass, Fergus J. Couch, Thomas Rohan, Iddo Z. Ben-Dov
Format: Article
Language:English
Published: MDPI AG 2017-03-01
Series:International Journal of Molecular Sciences
Subjects:
microRNAs
next-generation sequencing
cDNA library preparation
formalin-fixed paraffin-embedded
ductal carcinoma in situ
invasive breast cancer
Online Access:http://www.mdpi.com/1422-0067/18/3/627

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http://www.mdpi.com/1422-0067/18/3/627

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