An improved cerulean fluorescent protein with enhanced brightness and reduced reversible photoswitching.

An improved cerulean fluorescent protein with enhanced brightness and reduced reversible photoswitching.

Cyan fluorescent proteins (CFPs), such as Cerulean, are widely used as donor fluorophores in Förster resonance energy transfer (FRET) experiments. Nonetheless, the most widely used variants suffer from drawbacks that include low quantum yields and unstable flurorescence. To improve the fluorescence...

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Bibliographic Details
Main Authors: Michele L Markwardt, Gert-Jan Kremers, Catherine A Kraft, Krishanu Ray, Paula J C Cranfill, Korey A Wilson, Richard N Day, Rebekka M Wachter, Michael W Davidson, Mark A Rizzo
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-03-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3066204?pdf=render

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http://europepmc.org/articles/PMC3066204?pdf=render

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