Generation of a novel HEK293 luciferase reporter cell line by CRISPR/Cas9-mediated site-specific integration in the genome to explore the transcriptional regulation of the PGRN gene

Generation of a novel HEK293 luciferase reporter cell line by CRISPR/Cas9-mediated site-specific integration in the genome to explore the transcriptional regulation of the PGRN gene

Progranulin has multiple functions in several physiological and pathological processes, including embryonic development, wound repair, tumorigenesis, inflammation and neurodegeneration. To investigate the transcriptional regulation of the PGRN gene, a luciferase knock-in reporter system was establis...

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Bibliographic Details
Main Authors: Yanqing Li, Sai Li, Yan Li, Haibin Xia, Qinwen Mao
Format: Article
Language:English
Published: Taylor & Francis Group 2019-01-01
Series:Bioengineered
Subjects:
crispr/cas9
pgrn
knock-in
luciferase
transcriptional regulation
hek293
Online Access:http://dx.doi.org/10.1080/21655979.2019.1607126

Internet

http://dx.doi.org/10.1080/21655979.2019.1607126

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