Phasor-Based Endogenous NAD(P)H Fluorescence Lifetime Imaging Unravels Specific Enzymatic Activity of Neutrophil Granulocytes Preceding NETosis

Phasor-Based Endogenous NAD(P)H Fluorescence Lifetime Imaging Unravels Specific Enzymatic Activity of Neutrophil Granulocytes Preceding NETosis

Time-correlated single-photon counting combined with multi-photon laser scanning microscopy has proven to be a versatile tool to perform fluorescence lifetime imaging in biological samples and, thus, shed light on cellular functions, both in vitro and in vivo. Here, by means of phasor-analyzed endog...

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Bibliographic Details
Main Authors: Ruth Leben, Lennard Ostendorf, Sofie van Koppen, Asylkhan Rakhymzhan, Anja E. Hauser, Helena Radbruch, Raluca A. Niesner
Format: Article
Language:English
Published: MDPI AG 2018-03-01
Series:International Journal of Molecular Sciences
Subjects:
NAD(P)H (nicotinamide adenine dinucleotide (phosphate))
fluorescence lifetime imaging
neutrophil granulocytes
NADPH oxidase
NETosis
Online Access:http://www.mdpi.com/1422-0067/19/4/1018

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http://www.mdpi.com/1422-0067/19/4/1018

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