A sensitive S-Trap-based approach to the analysis of T cell lipid raft proteome

A sensitive S-Trap-based approach to the analysis of T cell lipid raft proteome

The analysis of T cell lipid raft proteome is challenging due to the highly dynamic nature of rafts and the hydrophobic character of raft-resident proteins. We explored an innovative strategy for bottom-up lipid raftomics based on suspension-trapping (S-Trap) sample preparation. Mouse T cells were p...

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Bibliographic Details
Main Authors: Cerina Chhuon, Shao-Yu Zhang, Vincent Jung, Daniel Lewandowski, Joanna Lipecka, André Pawlak, Dil Sahali, Mario Ollero, Ida Chiara Guerrera
Format: Article
Language:English
Published: Elsevier 2020-11-01
Series:Journal of Lipid Research
Subjects:
immunology
lymphocytes
membranes
tandem mass spectrometry
cell signaling
detergent-free
Online Access:http://www.sciencedirect.com/science/article/pii/S002222752043737X

Internet

http://www.sciencedirect.com/science/article/pii/S002222752043737X

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