Cisplatin-loaded mPEG-PCL Nanoparticles: Relation of PEG-PCL/PCL Hybrid Proportions and Antitumor Activity in Vitro
Objective To prepare cisplatin-loaded mPEG-PCL nanoparticles with different proportions of bi-block copolymer mPEG-PCL and PCL, and evaluate their antitumor effect in vitro. Methods mPEG-PCL and HO-PCL were synthesized by ring-opening polymerization method. Nanoparticles (NPs) prepared from mPEG-PCL...
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Magazine House of Cancer Research on Prevention and Treatment
2020-06-01
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doaj-70c69d6b617c4cd99deec114222445ad2020-11-25T02:56:33ZzhoMagazine House of Cancer Research on Prevention and TreatmentZhongliu Fangzhi Yanjiu1000-85781000-85782020-06-0147641141510.3971/j.issn.1000-8578.2020.19.09798578.2020.19.0979Cisplatin-loaded mPEG-PCL Nanoparticles: Relation of PEG-PCL/PCL Hybrid Proportions and Antitumor Activity in VitroWANG Chun0YU Lixia1WANG Lifeng2QIAN Hanqing3YAN Yingci4WANG Xinyue5LI Rutian6The Comprehensive Cancer Center of Drum-Tower Hospital, Medical School of Nanjing University & Clinical Cancer Institute of Nanjing University, Nanjing 210008, ChinaThe Comprehensive Cancer Center of Drum-Tower Hospital, Medical School of Nanjing University & Clinical Cancer Institute of Nanjing University, Nanjing 210008, ChinaThe Comprehensive Cancer Center of Drum-Tower Hospital, Medical School of Nanjing University & Clinical Cancer Institute of Nanjing University, Nanjing 210008, ChinaThe Comprehensive Cancer Center of Drum-Tower Hospital, Medical School of Nanjing University & Clinical Cancer Institute of Nanjing University, Nanjing 210008, ChinaThe Comprehensive Cancer Center of Drum-Tower Hospital, Medical School of Nanjing University & Clinical Cancer Institute of Nanjing University, Nanjing 210008, ChinaThe Comprehensive Cancer Center of Drum-Tower Hospital, Medical School of Nanjing University & Clinical Cancer Institute of Nanjing University, Nanjing 210008, ChinaThe Comprehensive Cancer Center of Drum-Tower Hospital, Medical School of Nanjing University & Clinical Cancer Institute of Nanjing University, Nanjing 210008, ChinaObjective To prepare cisplatin-loaded mPEG-PCL nanoparticles with different proportions of bi-block copolymer mPEG-PCL and PCL, and evaluate their antitumor effect in vitro. Methods mPEG-PCL and HO-PCL were synthesized by ring-opening polymerization method. Nanoparticles (NPs) prepared from mPEG-PCL/PCL hybrids were obtained by mixing PEG-PCL and PCL copolymer in different proportions. Drug-loading nanoparticles were prepared by w/o/w double emulsion method. The in vitro cytotoxicity of cisplatin-loaded nanoparticles on BGC-823 and SGC-7901 cell lines and the toxicity of the blank nanoparticles were evaluated by MTT assay. Results mPEG-PCL and PCL were synthesized with desired molecular weight. In vitro experiments showed the five kinds of nanoparticles had different antitumor activities and consistency in biocompatibility. Conclusion The properties of nanoparticles, such as diameters and drug loading capacities, vary with different ratio of components as well as the antitumor effect. The optimal hybrid proportions should be selected for further study and application.http://html.rhhz.net/ZLFZYJ/html/8578.2020.19.0979.htmcisplatinnanoparticlesw/o/w double-emulsion methoddrug-delivery systemantitumor |
collection |
DOAJ |
language |
zho |
format |
Article |
sources |
DOAJ |
author |
WANG Chun YU Lixia WANG Lifeng QIAN Hanqing YAN Yingci WANG Xinyue LI Rutian |
spellingShingle |
WANG Chun YU Lixia WANG Lifeng QIAN Hanqing YAN Yingci WANG Xinyue LI Rutian Cisplatin-loaded mPEG-PCL Nanoparticles: Relation of PEG-PCL/PCL Hybrid Proportions and Antitumor Activity in Vitro Zhongliu Fangzhi Yanjiu cisplatin nanoparticles w/o/w double-emulsion method drug-delivery system antitumor |
author_facet |
WANG Chun YU Lixia WANG Lifeng QIAN Hanqing YAN Yingci WANG Xinyue LI Rutian |
author_sort |
WANG Chun |
title |
Cisplatin-loaded mPEG-PCL Nanoparticles: Relation of PEG-PCL/PCL Hybrid Proportions and Antitumor Activity in Vitro |
title_short |
Cisplatin-loaded mPEG-PCL Nanoparticles: Relation of PEG-PCL/PCL Hybrid Proportions and Antitumor Activity in Vitro |
title_full |
Cisplatin-loaded mPEG-PCL Nanoparticles: Relation of PEG-PCL/PCL Hybrid Proportions and Antitumor Activity in Vitro |
title_fullStr |
Cisplatin-loaded mPEG-PCL Nanoparticles: Relation of PEG-PCL/PCL Hybrid Proportions and Antitumor Activity in Vitro |
title_full_unstemmed |
Cisplatin-loaded mPEG-PCL Nanoparticles: Relation of PEG-PCL/PCL Hybrid Proportions and Antitumor Activity in Vitro |
title_sort |
cisplatin-loaded mpeg-pcl nanoparticles: relation of peg-pcl/pcl hybrid proportions and antitumor activity in vitro |
publisher |
Magazine House of Cancer Research on Prevention and Treatment |
series |
Zhongliu Fangzhi Yanjiu |
issn |
1000-8578 1000-8578 |
publishDate |
2020-06-01 |
description |
Objective To prepare cisplatin-loaded mPEG-PCL nanoparticles with different proportions of bi-block copolymer mPEG-PCL and PCL, and evaluate their antitumor effect in vitro. Methods mPEG-PCL and HO-PCL were synthesized by ring-opening polymerization method. Nanoparticles (NPs) prepared from mPEG-PCL/PCL hybrids were obtained by mixing PEG-PCL and PCL copolymer in different proportions. Drug-loading nanoparticles were prepared by w/o/w double emulsion method. The in vitro cytotoxicity of cisplatin-loaded nanoparticles on BGC-823 and SGC-7901 cell lines and the toxicity of the blank nanoparticles were evaluated by MTT assay. Results mPEG-PCL and PCL were synthesized with desired molecular weight. In vitro experiments showed the five kinds of nanoparticles had different antitumor activities and consistency in biocompatibility. Conclusion The properties of nanoparticles, such as diameters and drug loading capacities, vary with different ratio of components as well as the antitumor effect. The optimal hybrid proportions should be selected for further study and application. |
topic |
cisplatin nanoparticles w/o/w double-emulsion method drug-delivery system antitumor |
url |
http://html.rhhz.net/ZLFZYJ/html/8578.2020.19.0979.htm |
work_keys_str_mv |
AT wangchun cisplatinloadedmpegpclnanoparticlesrelationofpegpclpclhybridproportionsandantitumoractivityinvitro AT yulixia cisplatinloadedmpegpclnanoparticlesrelationofpegpclpclhybridproportionsandantitumoractivityinvitro AT wanglifeng cisplatinloadedmpegpclnanoparticlesrelationofpegpclpclhybridproportionsandantitumoractivityinvitro AT qianhanqing cisplatinloadedmpegpclnanoparticlesrelationofpegpclpclhybridproportionsandantitumoractivityinvitro AT yanyingci cisplatinloadedmpegpclnanoparticlesrelationofpegpclpclhybridproportionsandantitumoractivityinvitro AT wangxinyue cisplatinloadedmpegpclnanoparticlesrelationofpegpclpclhybridproportionsandantitumoractivityinvitro AT lirutian cisplatinloadedmpegpclnanoparticlesrelationofpegpclpclhybridproportionsandantitumoractivityinvitro |
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1724713517951483904 |