Immobilized Horseradish Peroxidase on Discs of Polyvinyl Alcohol-Glutaraldehyde Coated with Polyaniline

Discs of network polyvinyl alcohol-glutaraldehyde (PVAG) were synthesized and coated with polyaniline (PANI) using glutaraldehyde as a chemical arm (PVAG-PANIG-HRP disc). The best conditions for the immobilization were established as about 1.0 mg mL−1 of protein, for 60 min and pH 5.5. The soluble e...

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Bibliographic Details
Main Authors: Samantha Salomão Caramori, Kátia Flávia Fernandes, Luiz Bezerra de Carvalho Junior
Format: Article
Language:English
Published: Hindawi Limited 2012-01-01
Series:The Scientific World Journal
Online Access:http://dx.doi.org/10.1100/2012/129706