In vivo hepatotoxicity screening of different extracts, components, and constituents of Polygoni Multiflori Thunb. in zebrafish (Danio rerio) larvae
Polygonum multiflorum Thunb. (PM) is a traditional Chinese medicine, commonly used to treat a variety of diseases. However, the hepatotoxicity associated with PM hampers its clinical application and development. In this study, we refined the zebrafish hepatotoxicity model with regard to the followin...
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Elsevier
2020-11-01
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Series: | Biomedicine & Pharmacotherapy |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S0753332220307174 |
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Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hong-Ying Li Jian-Bo Yang Wan-Fang Li Cai-Xia Qiu Guang Hu Shu-Ting Wang Yun-Fei Song Hui-Yu Gao Yue Liu Qi Wang Ying Wang Xian-Long Cheng Feng Wei Hong-Tao Jin Shuang-Cheng Ma |
spellingShingle |
Hong-Ying Li Jian-Bo Yang Wan-Fang Li Cai-Xia Qiu Guang Hu Shu-Ting Wang Yun-Fei Song Hui-Yu Gao Yue Liu Qi Wang Ying Wang Xian-Long Cheng Feng Wei Hong-Tao Jin Shuang-Cheng Ma In vivo hepatotoxicity screening of different extracts, components, and constituents of Polygoni Multiflori Thunb. in zebrafish (Danio rerio) larvae Biomedicine & Pharmacotherapy Zebrafish hepatotoxicity model Dianthrone Emodin Emodin-8-O-β-D-glucopyranoside Chrysophanol [Trans]-emodin-emodin dianthrone |
author_facet |
Hong-Ying Li Jian-Bo Yang Wan-Fang Li Cai-Xia Qiu Guang Hu Shu-Ting Wang Yun-Fei Song Hui-Yu Gao Yue Liu Qi Wang Ying Wang Xian-Long Cheng Feng Wei Hong-Tao Jin Shuang-Cheng Ma |
author_sort |
Hong-Ying Li |
title |
In vivo hepatotoxicity screening of different extracts, components, and constituents of Polygoni Multiflori Thunb. in zebrafish (Danio rerio) larvae |
title_short |
In vivo hepatotoxicity screening of different extracts, components, and constituents of Polygoni Multiflori Thunb. in zebrafish (Danio rerio) larvae |
title_full |
In vivo hepatotoxicity screening of different extracts, components, and constituents of Polygoni Multiflori Thunb. in zebrafish (Danio rerio) larvae |
title_fullStr |
In vivo hepatotoxicity screening of different extracts, components, and constituents of Polygoni Multiflori Thunb. in zebrafish (Danio rerio) larvae |
title_full_unstemmed |
In vivo hepatotoxicity screening of different extracts, components, and constituents of Polygoni Multiflori Thunb. in zebrafish (Danio rerio) larvae |
title_sort |
in vivo hepatotoxicity screening of different extracts, components, and constituents of polygoni multiflori thunb. in zebrafish (danio rerio) larvae |
publisher |
Elsevier |
series |
Biomedicine & Pharmacotherapy |
issn |
0753-3322 |
publishDate |
2020-11-01 |
description |
Polygonum multiflorum Thunb. (PM) is a traditional Chinese medicine, commonly used to treat a variety of diseases. However, the hepatotoxicity associated with PM hampers its clinical application and development. In this study, we refined the zebrafish hepatotoxicity model with regard to the following endpoints: liver size, liver gray value, and the area of yolk sac. The levels of alanine aminotransferase, aspartate transaminase, albumin, and microRNAs-122 were evaluated to verify the model. Subsequently, this model was used to screen different extracts, components, and constituents of PM, including 70 % EtOH extracts of PM, four fractions from macroporous resin (components A, B, C, and D), and 19 compounds from component D. We found that emodin, chrysophanol, emodin-8-O-β-D-glucopyranoside, (cis)-emodin-emodin dianthrones, and (trans)-emodin-emodin dianthrones showed higher hepatotoxicity compared to other components in PM, whereas polyphenols showed lower hepatotoxicity. To the best of our knowledge, this study is the first to identify that dianthrones may account for the hepatotoxicity of PM. We believe that these findings will be helpful in regulating the hepatotoxicity of PM. |
topic |
Zebrafish hepatotoxicity model Dianthrone Emodin Emodin-8-O-β-D-glucopyranoside Chrysophanol [Trans]-emodin-emodin dianthrone |
url |
http://www.sciencedirect.com/science/article/pii/S0753332220307174 |
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doaj-717b922db2494a60b3d37cc0bef62f9b2021-05-20T07:43:10ZengElsevierBiomedicine & Pharmacotherapy0753-33222020-11-01131110524In vivo hepatotoxicity screening of different extracts, components, and constituents of Polygoni Multiflori Thunb. in zebrafish (Danio rerio) larvaeHong-Ying Li0Jian-Bo Yang1Wan-Fang Li2Cai-Xia Qiu3Guang Hu4Shu-Ting Wang5Yun-Fei Song6Hui-Yu Gao7Yue Liu8Qi Wang9Ying Wang10Xian-Long Cheng11Feng Wei12Hong-Tao Jin13Shuang-Cheng Ma14New Drug Safety Evaluation Center, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100050, ChinaInstitute for Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing, 100050, ChinaNew Drug Safety Evaluation Center, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100050, ChinaNew Drug Safety Evaluation Center, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100050, ChinaNew Drug Safety Evaluation Center, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100050, ChinaNew Drug Safety Evaluation Center, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100050, ChinaInstitute for Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing, 100050, ChinaInstitute for Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing, 100050, ChinaInstitute for Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing, 100050, ChinaInstitute for Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing, 100050, ChinaInstitute for Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing, 100050, ChinaInstitute for Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing, 100050, ChinaInstitute for Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing, 100050, ChinaNew Drug Safety Evaluation Center, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100050, China; Beijing Union-Genius Pharmaceutical Technology Co. Ltd, Beijing, 100176, China; Corresponding author at: New Drug Safety Evaluation Center, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100050, China.Institute for Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing, 100050, China; Corresponding author.Polygonum multiflorum Thunb. (PM) is a traditional Chinese medicine, commonly used to treat a variety of diseases. However, the hepatotoxicity associated with PM hampers its clinical application and development. In this study, we refined the zebrafish hepatotoxicity model with regard to the following endpoints: liver size, liver gray value, and the area of yolk sac. The levels of alanine aminotransferase, aspartate transaminase, albumin, and microRNAs-122 were evaluated to verify the model. Subsequently, this model was used to screen different extracts, components, and constituents of PM, including 70 % EtOH extracts of PM, four fractions from macroporous resin (components A, B, C, and D), and 19 compounds from component D. We found that emodin, chrysophanol, emodin-8-O-β-D-glucopyranoside, (cis)-emodin-emodin dianthrones, and (trans)-emodin-emodin dianthrones showed higher hepatotoxicity compared to other components in PM, whereas polyphenols showed lower hepatotoxicity. To the best of our knowledge, this study is the first to identify that dianthrones may account for the hepatotoxicity of PM. We believe that these findings will be helpful in regulating the hepatotoxicity of PM.http://www.sciencedirect.com/science/article/pii/S0753332220307174Zebrafish hepatotoxicity modelDianthroneEmodinEmodin-8-O-β-D-glucopyranosideChrysophanol[Trans]-emodin-emodin dianthrone |