Microfluidic PCR Amplification and MiSeq Amplicon Sequencing Techniques for High-Throughput Detection and Genotyping of Human Pathogenic RNA Viruses in Human Feces, Sewage, and Oysters

Microfluidic PCR Amplification and MiSeq Amplicon Sequencing Techniques for High-Throughput Detection and Genotyping of Human Pathogenic RNA Viruses in Human Feces, Sewage, and Oysters

Detection and genotyping of pathogenic RNA viruses in human and environmental samples are useful for monitoring the circulation and prevalence of these pathogens, whereas a conventional PCR assay followed by Sanger sequencing is time-consuming and laborious. The present study aimed to develop a high...

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Bibliographic Details
Main Authors: Mamoru Oshiki, Takayuki Miura, Shinobu Kazama, Takahiro Segawa, Satoshi Ishii, Masashi Hatamoto, Takashi Yamaguchi, Kengo Kubota, Akinori Iguchi, Tadashi Tagawa, Tsutomu Okubo, Shigeki Uemura, Hideki Harada, Naohiro Kobayashi, Nobuo Araki, Daisuke Sano
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-04-01
Series:Frontiers in Microbiology
Subjects:
human pathogenic viruses
high-throughput detection and genotyping
microfluidic PCR
MiSeq sequencing
sewage
human feces
Online Access:http://journal.frontiersin.org/article/10.3389/fmicb.2018.00830/full

Internet

http://journal.frontiersin.org/article/10.3389/fmicb.2018.00830/full

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